STP22/YCL008C Protein Information

Standard Name	Stp22p
Systematic Name	Ycl008cp
Alias	Vps23p 1, 2 , Vpl15p 2
ORF Classification	Verified
Description	Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype (3, 4)
Name Description	STerile Pseudoreversion 5

Experimental Data
Molecules/cell	1360 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	385
Molecular Weight (Da)	43,330
Isoelectric Point (pI)	5.53

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Stp22p (InterPro)
Physical Interactions	There are 79 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000699FB \| P25604 MIPS: YCL008C NCBI: 14588921 \| 149242807 \| 2252812 \| 30267750 \| 332138262 \| 332138283 \| 37999925 \| 42759852 \| 47169416 \| 99031926 \| 99031929 \| 99032188 \| 99032191 \| 99032194 \| 99032196 \| NP_009919.3 \| NM_001178657.1 GenBank/EMBL/DDBJ: DAA07473.1 \| AF004731 \| AY260880 \| X59720

Amino Acid Sequence (or in FASTA format)
1 MSANGKISVP EAVVNWLFKV IQPIYNDGRT TFHDSLALLD NFHSLRPRTR 51 VFTHSDGTPQ LLLSIYGTIS TGEDGSSPHS IPVIMWVPSM YPVKPPFISI 101 NLENFDMNTI SSSLPIQEYI DSNGWIALPI LHCWDPAAMN LIMVVQELMS 151 LLHEPPQDQA PSLPPKPNTQ LQQEQNTPPL PPKPKSPHLK PPLPPPPPPQ 201 PASNALDLMD MDNTDISPTN HHEMLQNLQT VVNELYREDV DYVADKILTR 251 QTVMQESIAR FHEIIAIDKN HLRAVEQAIE QTMHSLNAQI DVLTANRAKV 301 QQFSSTSHVD DEDVNSIAVA KTDGLNQLYN LVAQDYALTD TIECLSRMLH 351 RGTIPLDTFV KQGRELARQQ FLVRWHIQRI TSPLS*

Amino Acid Sequence (or in FASTA format)

       1  MSANGKISVP EAVVNWLFKV IQPIYNDGRT TFHDSLALLD NFHSLRPRTR
      51  VFTHSDGTPQ LLLSIYGTIS TGEDGSSPHS IPVIMWVPSM YPVKPPFISI
     101  NLENFDMNTI SSSLPIQEYI DSNGWIALPI LHCWDPAAMN LIMVVQELMS
     151  LLHEPPQDQA PSLPPKPNTQ LQQEQNTPPL PPKPKSPHLK PPLPPPPPPQ
     201  PASNALDLMD MDNTDISPTN HHEMLQNLQT VVNELYREDV DYVADKILTR
     251  QTVMQESIAR FHEIIAIDKN HLRAVEQAIE QTMHSLNAQI DVLTANRAKV
     301  QQFSSTSHVD DEDVNSIAVA KTDGLNQLYN LVAQDYALTD TIECLSRMLH
     351  RGTIPLDTFV KQGRELARQQ FLVRWHIQRI TSPLS*

external links for Stp22p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Stp22p

1)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3)	Babst M, et al. (2000) Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking. Traffic 1(3):248-58
4)	Katzmann DJ, et al. (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55
5)	Li Y, et al. (1999) Yeast mutants affecting possible quality control of plasma membrane proteins. Mol Cell Biol 19(5):3588-99
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41