DUG2/YBR281C Protein Information Help

Standard Name Dug2p 1
Systematic Name Ybr281cp
ORF Classification Verified
Description Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) (1, 2)
Name Description Deficient in Utilization of Glutathione 1
Experimental Data
Molecules/cell 1940 3
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 878
Molecular Weight (Da) 98,070
Isoelectric Point (pI) 5.3

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dug2p (InterPro)
Physical Interactions There are 18 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013A2A3 | P38149
MIPS: YBR281C
NCBI: 398365863 | 429126 | 536726 | 586394 | NP_009840.3
GenBank/EMBL/DDBJ: DAA07396.1 | X76053 | Z36150
External Classifications EC: 3.4.-.- [Peptide hydrolases]
Amino Acid Sequence (or in FASTA format)
 
       1  MYDSRGVALH SELIHRWNHA FSILSIVAFP KKRLLFAGSQ DSKILVFDLP
      51  TYNLIHTIRL GESQEETHTR SSVLCLTGSE DENFLFSGGA DSLVRIWSIG
     101  EKTIRDDFLP VTEIATVYSV TDIGDIFSLA YLDSLETIVF GCQNASLLYV
     151  ENLIQKIEKK SSDGVENINK LPHRRYDKFF DSLGPTGYSS NSLSQTSLTS
     201  LQENCGAAII EVPSENIIKY AHYGFIYSIN KLCPRFNQLL EKSSRTSGAE
     251  HIISSAGDGI SKLWEFSKDK GQNTVKISLI NDKIDNEDSV ISQTIEFPFL
     301  YCGLTDGIIK IWDLNTQQII STLKTKHESD VISISVYMDH VFAIDESGIT
     351  HFYQNQVNHW NPQQGKILSS EIFSKSNAGS VSLLTGGSDG SLTLWDITSL
     401  LSAVPLSSNS PINASSTLQT TNLWAAYQSA SLNNEEMLNT LRELISFQTV
     451  SQSKDTTNTL SLRRCAIYLQ QLFLKFGATN SQLFPLPDGG NPVVFAYFQG
     501  NGKVSQVKGA KKKRILWYGH YDVISSGNTF NWNTDPFTLT CENGYLKGRG
     551  VSDNKGPLVS AIHSVAYLFQ QGELVNDVVF LVEGSEEIGS ASLKQVCEKY
     601  HDIIGKDIDW ILLSNSTWVD QEHPCLNYGL RGVINAQIKV WSDKPDGHSG
     651  LNGGVYDEPM VNLVKIVSKL QNEQNEIMIP NFYSPLKDLT EEEYQRFQKI
     701  TELANIDENT TVQDLITNWT KPSLSMTTVK FSGPGNITVI PKSVTMGISI
     751  RLVPEQSVEQ VKRDLKAYLE ESFKQLKSQN HLEIKVLNEA EGWLGDPTNH
     801  AYQILKDEIT TAWDVEPLLV REGGSISCLR MLERIFDAPA VQIPCGQSTD
     851  NGHLANENLR IKNWSNLTEI LSKVFNRL*

external links for Dug2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Dug2p
1) Ganguli D, et al.  (2007) The Alternative Pathway of Glutathione Degradation Is Mediated by a Novel Protein Complex Involving Three New Genes in Saccharomyces cerevisiae. Genetics 175(3):1137-51
2) Kaur H, et al.  (2012) Glutathione degradation by the alternative pathway (DUG pathway) in Saccharomyces cerevisiae is initiated by (Dug2p-Dug3p)2 complex, a novel glutamine amidotransferase (GATase) enzyme acting on glutathione. J Biol Chem 287(12):8920-31
3) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41