PAF1/YBR279W Protein Information

Standard Name	Paf1p 1
Systematic Name	Ybr279wp
ORF Classification	Verified
Description	Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 (2, 3, 4, 5, 6, 7)
Name Description	RNA Polymerase II Associated Factor 1

Experimental Data
Molecules/cell	1040 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	445
Molecular Weight (Da)	51,800
Isoelectric Point (pI)	4.63

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Paf1p (InterPro)
Physical Interactions	There are 125 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053037 \| P38351 MIPS: YBR279W NCBI: 429124 \| 536722 \| 586392 \| 6319756 \| NP_009838.1 GenBank/EMBL/DDBJ: DAA07394.1 \| X76053 \| Z36148

Amino Acid Sequence (or in FASTA format)
1 MSKKQEYIAP IKYQNSLPVP QLPPKLLVYP ESPETNADSS QLINSLYIKT 51 NVTNLIQQDE DLGMPVDLMK FPGLLNKLDS KLLYGFDNVK LDKDDRILLR 101 DPRIDRLTKT DISKVTFLRR TEYVSNTIAA HDNTSLKRKR RLDDGDSDDE 151 NLDVNHIISR VEGTFNKTDK WQHPVKKGVK MVKKWDLLPD TASMDQVYFI 201 LKFMGSASLD TKEKKSLNTG IFRPVELEED EWISMYATDH KDSAILENEL 251 EKGMDEMDDD SHEGKIYKFK RIRDYDMKQV AEKPMTELAI RLNDKDGIAY 301 YKPLRSKIEL RRRRVNDIIK PLVKEHDIDQ LNVTLRNPST KEANIRDKLR 351 MKFDPINFAT VDEEDDEDEE QPEDVKKESE GDSKTEGSEQ EGENEKDEEI 401 KQEKENEQDE ENKQDENRAA DTPETSDAVH TEQKPEEEKE TLQEE*

Amino Acid Sequence (or in FASTA format)

       1  MSKKQEYIAP IKYQNSLPVP QLPPKLLVYP ESPETNADSS QLINSLYIKT
      51  NVTNLIQQDE DLGMPVDLMK FPGLLNKLDS KLLYGFDNVK LDKDDRILLR
     101  DPRIDRLTKT DISKVTFLRR TEYVSNTIAA HDNTSLKRKR RLDDGDSDDE
     151  NLDVNHIISR VEGTFNKTDK WQHPVKKGVK MVKKWDLLPD TASMDQVYFI
     201  LKFMGSASLD TKEKKSLNTG IFRPVELEED EWISMYATDH KDSAILENEL
     251  EKGMDEMDDD SHEGKIYKFK RIRDYDMKQV AEKPMTELAI RLNDKDGIAY
     301  YKPLRSKIEL RRRRVNDIIK PLVKEHDIDQ LNVTLRNPST KEANIRDKLR
     351  MKFDPINFAT VDEEDDEDEE QPEDVKKESE GDSKTEGSEQ EGENEKDEEI
     401  KQEKENEQDE ENKQDENRAA DTPETSDAVH TEQKPEEEKE TLQEE*

external links for Paf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Paf1p

1)	Wade PA, et al. (1996) A novel collection of accessory factors associated with yeast RNA polymerase II. Protein Expr Purif 8(1):85-90
2)	Mueller CL and Jaehning JA (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
3)	Betz JL, et al. (2002) Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism. Mol Genet Genomics 268(2):272-85
4)	Moniaux N, et al. (2006) The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis. Oncogene 25(23):3247-57
5)	Zhang Y, et al. (2009) The Paf1 complex is required for efficient transcription elongation by RNA polymerase I. Proc Natl Acad Sci U S A 106(7):2153-8
6)	Tous C, et al. (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
7)	Pruneski JA, et al. (2011) The Paf1 complex represses SER3 transcription in Saccharomyces cerevisiae by facilitating intergenic transcription-dependent nucleosome occupancy of the SER3 promoter. Eukaryot Cell 10(10):1283-94
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41