RIF1/YBR275C Protein Information

Standard Name	Rif1p 1
Systematic Name	Ybr275cp
ORF Classification	Verified
Description	Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation (2, 3, 4)
Name Description	RAP1-Interacting Factor 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,916
Molecular Weight (Da)	217,930
Isoelectric Point (pI)	6.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rif1p (InterPro)
Physical Interactions	There are 20 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD39 \| P29539 MIPS: YBR275C NCBI: 1870116 \| 341942151 \| 398365841 \| 429120 \| 4328 \| 536715 \| 585855 \| NP_009834.4 GenBank/EMBL/DDBJ: DAA07391.2 \| X66501 \| X76053 \| Z36144 \| Z36145

Amino Acid Sequence (or in FASTA format)
1 MSKDFSDKKK HTIDRIDQHI LRRSQHDNYS NGSSPWMKTN LPPPSPQAHM 51 HIQSDLSPTP KRRKLASSSD CENKQFDLSA INKNLYPEDT GSRLMQSLPE 101 LSASNSDNVS PVTKSVAFSD RIESSPIYRI PGSSPKPSPS SKPGKSILRN 151 RLPSVRTVSD LSYNKLQYTQ HKLHNGNIFT SPYKETRVNP RALEYWVSGE 201 IHGLVDNESV SEFKEIIEGG LGILRQESED YVARRFEVYA TFNNIIPILT 251 TKNVNEVDQK FNILIVNIES IIEICIPHLQ IAQDTLLSSS EKKNPFVIRL 301 YVQIVRFFSA IMSNFKIVKW LTKRPDLVNK LKVIYRWTTG ALRNENSNKI 351 IITAQVSFLR DEKFGTFFLS NEEIKPIIST FTEIMEINSH NLIYEKLLLI 401 RGFLSKYPKL MIETVTSWLP GEVLPRIIIG DEIYSMKILI TSIVVLLELL 451 KKCLDFVDEH ERIYQCIMLS PVCETIPEKF LSKLPLNSYD SANLDKVTIG 501 HLLTQQIKNY IVVKNDNKIA MDLWLSMTGL LYDSGKRVYD LTSESNKVWF 551 DLNNLCFINN HPKTRLMSIK VWRIITYCIC TKISQKNQEG NKSLLSLLRT 601 PFQMTLPYVN DPSAREGIIY HLLGVVYTAF TSNKNLSTDM FELFWDHLIT 651 PIYEDYVFKY DSIHLQNVLF TVLHLLIGGK NADVALERKY KKHIHPMSVI 701 ASEGVKLKDI SSLPPQIIKR EYDKIMKVVF QAVEVAISNV NLAHDLILTS 751 LKHLPEDRKD QTHLESFSSL ILKVTQNNKD TPIFRDFFGA VTSSFVYTFL 801 DLFLRKNDSS LVNFNIQISK VGISQGNMTL DLLKDVIRKA RNETSEFLII 851 EKFLELDDKK TEVYAQNWVG STLLPPNISF REFQSLANIV NKVPNENSIE 901 NFLDLCLKLS FPVNLFTLLH VSMWSNNNFI YFIQSYVSKN ENKLNVDLIT 951 LLKTSLPGNP ELFSGLLPFL RRNKFMDILE YCIHSNPNLL NSIPDLNSDL 1001 LLKLLPRSRA SYFAANIKLF KCSEQLTLVR WLLKGQQLEQ LNQNFSEIEN 1051 VLQNASDSEL EKSEIIRELL HLAMANPIEP LFSGLLNFCI KNNMADHLDE 1101 FCGNMTSEVL FKISPELLLK LLTYKEKPNG KLLAAVIEKI ENGDDDYILE 1151 LLEKIIIQKE IQILEKLKEP LLVFFLNPVS SNMQKHKKST NMLRELVLLY 1201 LTKPLSRSAA KKFFSMLISI LPPNPNYQTI DMVNLLIDLI KSHNRKFKDK 1251 RTYNATLKTI GKWIQESGVV HQGDSSKEIE AIPDTKSMYI PCEGSENKLS 1301 NLQRKVDSQD IQVPATQGMK EPPSSIQISS QISAKDSDSI SLKNTAIMNS 1351 SQQESHANRS RSIDDETLEE VDNESIREID QQMKSTQLDK NVANHSNICS 1401 TKSDEVDVTE LHESIDTQSS EVNAYQPIEV LTSELKAVTN RSIKTNPDHN 1451 VVNSDNPLKR PSKETPTSEN KRSKGHETMV DVLVSEEQAV SPSSDVICTN 1501 IKSIANEESS LALRNSIKVE TNCNENSLNV TLDLDQQTIT KEDGKGQVEH 1551 VQRQENQESM NKINSKSFTQ DNIAQYKSVK KARPNNEGEN NDYACNVEQA 1601 SPVRNEVPGD GIQIPSGTIL LNSSKQTEKS KVDDLRSDED EHGTVAQEKH 1651 QVGAINSRNK NNDRMDSTPI QGTEEESREV VMTEEGINVR LEDSGTCELN 1701 KNLKGPLKGD KDANINDDFV PVEENVRDEG FLKSMEHAVS KETGLEEQPE 1751 VADISVLPEI RIPIFNSLKM QGSKSQIKEK LKKRLQRNEL MPPDSPPRMT 1801 ENTNINAQNG LDTVPKTIGG KEKHHEIQLG QAHTEADGEP LLGGDGNEDA 1851 TSREATPSLK VHFFSKKSRR LVARLRGFTP GDLNGISVEE RRNLRIELLD 1901 FMMRLEYYSN RDNDMN*

Amino Acid Sequence (or in FASTA format)

       1  MSKDFSDKKK HTIDRIDQHI LRRSQHDNYS NGSSPWMKTN LPPPSPQAHM
      51  HIQSDLSPTP KRRKLASSSD CENKQFDLSA INKNLYPEDT GSRLMQSLPE
     101  LSASNSDNVS PVTKSVAFSD RIESSPIYRI PGSSPKPSPS SKPGKSILRN
     151  RLPSVRTVSD LSYNKLQYTQ HKLHNGNIFT SPYKETRVNP RALEYWVSGE
     201  IHGLVDNESV SEFKEIIEGG LGILRQESED YVARRFEVYA TFNNIIPILT
     251  TKNVNEVDQK FNILIVNIES IIEICIPHLQ IAQDTLLSSS EKKNPFVIRL
     301  YVQIVRFFSA IMSNFKIVKW LTKRPDLVNK LKVIYRWTTG ALRNENSNKI
     351  IITAQVSFLR DEKFGTFFLS NEEIKPIIST FTEIMEINSH NLIYEKLLLI
     401  RGFLSKYPKL MIETVTSWLP GEVLPRIIIG DEIYSMKILI TSIVVLLELL
     451  KKCLDFVDEH ERIYQCIMLS PVCETIPEKF LSKLPLNSYD SANLDKVTIG
     501  HLLTQQIKNY IVVKNDNKIA MDLWLSMTGL LYDSGKRVYD LTSESNKVWF
     551  DLNNLCFINN HPKTRLMSIK VWRIITYCIC TKISQKNQEG NKSLLSLLRT
     601  PFQMTLPYVN DPSAREGIIY HLLGVVYTAF TSNKNLSTDM FELFWDHLIT
     651  PIYEDYVFKY DSIHLQNVLF TVLHLLIGGK NADVALERKY KKHIHPMSVI
     701  ASEGVKLKDI SSLPPQIIKR EYDKIMKVVF QAVEVAISNV NLAHDLILTS
     751  LKHLPEDRKD QTHLESFSSL ILKVTQNNKD TPIFRDFFGA VTSSFVYTFL
     801  DLFLRKNDSS LVNFNIQISK VGISQGNMTL DLLKDVIRKA RNETSEFLII
     851  EKFLELDDKK TEVYAQNWVG STLLPPNISF REFQSLANIV NKVPNENSIE
     901  NFLDLCLKLS FPVNLFTLLH VSMWSNNNFI YFIQSYVSKN ENKLNVDLIT
     951  LLKTSLPGNP ELFSGLLPFL RRNKFMDILE YCIHSNPNLL NSIPDLNSDL
    1001  LLKLLPRSRA SYFAANIKLF KCSEQLTLVR WLLKGQQLEQ LNQNFSEIEN
    1051  VLQNASDSEL EKSEIIRELL HLAMANPIEP LFSGLLNFCI KNNMADHLDE
    1101  FCGNMTSEVL FKISPELLLK LLTYKEKPNG KLLAAVIEKI ENGDDDYILE
    1151  LLEKIIIQKE IQILEKLKEP LLVFFLNPVS SNMQKHKKST NMLRELVLLY
    1201  LTKPLSRSAA KKFFSMLISI LPPNPNYQTI DMVNLLIDLI KSHNRKFKDK
    1251  RTYNATLKTI GKWIQESGVV HQGDSSKEIE AIPDTKSMYI PCEGSENKLS
    1301  NLQRKVDSQD IQVPATQGMK EPPSSIQISS QISAKDSDSI SLKNTAIMNS
    1351  SQQESHANRS RSIDDETLEE VDNESIREID QQMKSTQLDK NVANHSNICS
    1401  TKSDEVDVTE LHESIDTQSS EVNAYQPIEV LTSELKAVTN RSIKTNPDHN
    1451  VVNSDNPLKR PSKETPTSEN KRSKGHETMV DVLVSEEQAV SPSSDVICTN
    1501  IKSIANEESS LALRNSIKVE TNCNENSLNV TLDLDQQTIT KEDGKGQVEH
    1551  VQRQENQESM NKINSKSFTQ DNIAQYKSVK KARPNNEGEN NDYACNVEQA
    1601  SPVRNEVPGD GIQIPSGTIL LNSSKQTEKS KVDDLRSDED EHGTVAQEKH
    1651  QVGAINSRNK NNDRMDSTPI QGTEEESREV VMTEEGINVR LEDSGTCELN
    1701  KNLKGPLKGD KDANINDDFV PVEENVRDEG FLKSMEHAVS KETGLEEQPE
    1751  VADISVLPEI RIPIFNSLKM QGSKSQIKEK LKKRLQRNEL MPPDSPPRMT
    1801  ENTNINAQNG LDTVPKTIGG KEKHHEIQLG QAHTEADGEP LLGGDGNEDA
    1851  TSREATPSLK VHFFSKKSRR LVARLRGFTP GDLNGISVEE RRNLRIELLD
    1901  FMMRLEYYSN RDNDMN*

external links for Rif1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rif1p

1)	Hardy CF, et al. (1992) A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes Dev 6(5):801-14
2)	Wotton D and Shore D (1997) A novel Rap1p-interacting factor, Rif2p, cooperates with Rif1p to regulate telomere length in Saccharomyces cerevisiae. Genes Dev 11(6):748-60
3)	Teixeira MT, et al. (2004) Telomere length homeostasis is achieved via a switch between telomerase- extendible and -nonextendible states. Cell 117(3):323-35
4)	Smogorzewska A and de Lange T (2004) Regulation of telomerase by telomeric proteins. Annu Rev Biochem 73:177-208