CHK1/YBR274W Protein Information Help

Standard Name Chk1p 1
Systematic Name Ybr274wp
ORF Classification Verified
Description Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase (1, 2, 3, 4, 5)
Name Description CHeckpoint Kinase 1, 6
Experimental Data
Molecules/cell 2530 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 527
Molecular Weight (Da) 60,016
Isoelectric Point (pI) 6.21

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Chk1p (InterPro)
Physical Interactions There are 55 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127836 | P38147
MIPS: YBR274W
NCBI: 4325043 | 536713 | 585344 | 6319751 | NP_009833.1 | NM_001178622.1
GenBank/EMBL/DDBJ: DAA07390.1 | AF117345 | Z36143
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP
      51  TCKKMGLSDK DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG
     101  GDLFDKIEPD VGVDSDVAQF YFQQLVSAIN YLHVECGVAH RDIKPENILL
     151  DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ RGSPPYMAPE VLYSEEGYYA
     201  DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG NLNWGPWSKI
     251  EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL
     301  AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF
     351  QTVSNTQRAF TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND
     401  CNELVKRHLQ FNPNKLTKFY TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN
     451  FERLCELLGY DNVFPLIINI KTKSNGGYQL CGSISIIKIE EELKSVGFER
     501  KTGDPLEWRR LFKKISTICR DIILIPN*

external links for Chk1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
YGOB CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YOGY Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Chk1p
1) Liu Y, et al.  (2000) Characterization of a Saccharomyces cerevisiae homologue of Schizosaccharomyces pombe Chk1 involved in DNA-damage-induced M-phase arrest. Mol Gen Genet 262(6):1132-46
2) Wang H, et al.  (2001) Pds1 phosphorylation in response to DNA damage is essential for its DNA damage checkpoint function. Genes Dev 15(11):1361-72
3) Rouse J and Jackson SP  (2002) Interfaces between the detection, signaling, and repair of DNA damage. Science 297(5581):547-51
4) Agarwal R, et al.  (2003) Two distinct pathways for inhibiting pds1 ubiquitination in response to DNA damage. J Biol Chem 278(45):45027-33
5) Chen Y and Sanchez Y  (2004) Chk1 in the DNA damage response: conserved roles from yeasts to mammals. DNA Repair (Amst) 3(8-9):1025-32
6) Sanchez Y, et al.  (1999) Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms. Science 286(5442):1166-71
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41