HSM3/YBR272C Protein Information Help

Standard Name Hsm3p
Systematic Name Ybr272cp
ORF Classification Verified
Description Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation (1, 2, 3, 4, 5, 6, 7)
Name Description enHanced Spontaneous Mutability 8
Experimental Data
Molecules/cell 468 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 480
Molecular Weight (Da) 55,542
Isoelectric Point (pI) 4.55

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hsm3p (InterPro)
Physical Interactions There are 76 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000530B9 | P38348
MIPS: YBR272C
NCBI: 377656525 | 377656526 | 377656527 | 377656528 | 377656529 | 377656531 | 383875662 | 383875663 | 383875664 | 383875666 | 398365825 | 51013101 | 536709 | 586388 | NP_009831.3
GenBank/EMBL/DDBJ: DAA07388.1 | AY692825 | Z36141
Amino Acid Sequence (or in FASTA format)
 
       1  MSEKETNYVE NLLTQLENEL NEDNLPEDIN TLLRKCSLNL VTVVSLPDMD
      51  VKPLLATIKR FLTSNVSYDS LNYDYLLDVV DKLVPMADFD DVLEVYSAED
     101  LVKALRSEID PLKVAACRVI ENSQPKGLFA TSNIIDILLD ILFDEKVEND
     151  KLITAIEKAL ERLSTDELIR RRLFDNNLPY LVSVKGRMET VSFVRLIDFL
     201  TIEFQFISGP EFKDIIFCFT KEEILKSVED ILVFIELVNY YTKFLLEIRN
     251  QDKYWALRHV KKILPVFAQL FEDTENYPDV RAFSTNCLLQ LFAEVSRIEE
     301  DEYSLFKTMD KDSLKIGSEA KLITEWLELI NPQYLVKYHK DVVENYFHVS
     351  GYSIGMLRNL SADEECFNAI RNKFSAEIVL RLPYLEQMQV VETLTRYEYT
     401  SKFLLNEMPK VMGSLIGDGS AGAIIDLETV HYRNSALRNL LDKGEEKLSV
     451  WYEPLLREYS KAVNGKNYST GSETKIADCR *

external links for Hsm3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
YGOB CYC2008 (complexes) Pfam domains
YOGY Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Hsm3p
1) Merker JD, et al.  (2000) The yeast HSM3 gene is not involved in DNA mismatch repair in rapidly dividing cells. Genetics 154(1):491-3
2) Fedorova IV, et al.  (2000) The yeast HSM3 gene is involved in DNA mismatch repair in slowly dividing cells. Genetics 154(1):495-6
3) Le Tallec B, et al.  (2009) Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome. Mol Cell 33(3):389-99
4) Roelofs J, et al.  (2009) Chaperone-mediated pathway of proteasome regulatory particle assembly. Nature 459(7248):861-5
5) Saeki Y, et al.  (2009) Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. Cell 137(5):900-13
6) Funakoshi M, et al.  (2009) Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell 137(5):887-99
7) Takagi K, et al.  (2012) Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p. J Biol Chem 287(15):12172-82
8) Fedorova IV, et al.  (1998) The yeast HSM3 gene acts in one of the mismatch repair pathways. Genetics 148(3):963-73
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41