ARO4/YBR249C Protein Information

Standard Name	Aro4p
Systematic Name	Ybr249cp
ORF Classification	Verified
Description	3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4)
Name Description	AROmatic amino acid requiring

Experimental Data
Molecules/cell	26300 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	370
Molecular Weight (Da)	39,749
Isoelectric Point (pI)	6.93

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Aro4p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000111B42 \| P32449 MIPS: YBR249C NCBI: 240104642 \| 240104643 \| 240104644 \| 240104645 \| 240104646 \| 240104647 \| 240104648 \| 240104649 \| 240104650 \| 240104651 \| 240104652 \| 28373350 \| 28373351 \| 28373352 \| 28373353 \| 28373354 \| 28373355 \| 28373356 \| 28373357 \| 311102 \| 42543144 \| 42543145 \| 433966 \| 461540 \| 48425085 \| 48425086 \| 48425087 \| 48425088 \| 48425089 \| 48425090 \| 48425094 \| 48425095 \| 48425096 \| 48425097 \| 48425098 \| 48425099 \| 48425100 \| 48425101 \| 48425102 \| 48425103 \| 48425104 \| 48425105 \| 48425108 \| 48425109 \| 48425113 \| 49258345 \| 49258346 \| 49258347 \| 49258348 \| 49258349 \| 49258350 \| 49258351 \| 49258352 \| 536665 \| 6319726 \| NP_009808.1 GenBank/EMBL/DDBJ: DAA07365.1 \| L20296 \| X61107 \| Z36118
External Classifications	EC: 2.5.1.54 [3-deoxy-7-phosphoheptulonate synthase]

Amino Acid Sequence (or in FASTA format)
1 MSESPMFAAN GMPKVNQGAE EDVRILGYDP LASPALLQVQ IPATPTSLET 51 AKRGRREAID IITGKDDRVL VIVGPCSIHD LEAAQEYALR LKKLSDELKG 101 DLSIIMRAYL EKPRTTVGWK GLINDPDVNN TFNINKGLQS ARQLFVNLTN 151 IGLPIGSEML DTISPQYLAD LVSFGAIGAR TTESQLHREL ASGLSFPVGF 201 KNGTDGTLNV AVDACQAAAH SHHFMGVTKH GVAAITTTKG NEHCFVILRG 251 GKKGTNYDAK SVAEAKAQLP AGSNGLMIDY SHGNSNKDFR NQPKVNDVVC 301 EQIANGENAI TGVMIESNIN EGNQGIPAEG KAGLKYGVSI TDACIGWETT 351 EDVLRKLAAA VRQRREVNKK *

Amino Acid Sequence (or in FASTA format)

       1  MSESPMFAAN GMPKVNQGAE EDVRILGYDP LASPALLQVQ IPATPTSLET
      51  AKRGRREAID IITGKDDRVL VIVGPCSIHD LEAAQEYALR LKKLSDELKG
     101  DLSIIMRAYL EKPRTTVGWK GLINDPDVNN TFNINKGLQS ARQLFVNLTN
     151  IGLPIGSEML DTISPQYLAD LVSFGAIGAR TTESQLHREL ASGLSFPVGF
     201  KNGTDGTLNV AVDACQAAAH SHHFMGVTKH GVAAITTTKG NEHCFVILRG
     251  GKKGTNYDAK SVAEAKAQLP AGSNGLMIDY SHGNSNKDFR NQPKVNDVVC
     301  EQIANGENAI TGVMIESNIN EGNQGIPAEG KAGLKYGVSI TDACIGWETT
     351  EDVLRKLAAA VRQRREVNKK *

external links for Aro4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Aro4p

1)	Kunzler M, et al. (1992) Cloning, primary structure and regulation of the ARO4 gene, encoding the tyrosine-inhibited 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae. Gene 113(1):67-74
2)	Jones EW and Fink GR (1982) "Regulation of amino acid and nucleotide biosynthesis in yeast." Pp.181-299 in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, edited by Strathern JN, Jones EW and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
3)	Helmstaedt K, et al. (2005) Evolution of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase-encoding genes in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102(28):9784-9
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41