RRT2/YBR246W Protein Information

Standard Name	Rrt2p 1
Systematic Name	Ybr246wp
Alias	Ere1p 2 , Dph7p 3, 4
ORF Classification	Verified
Description	Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription (1, 2, 4, 5, 6)
Name Description	Regulator of rDNA Transcription 1

Experimental Data
Molecules/cell	2640 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	387
Molecular Weight (Da)	43,308
Isoelectric Point (pI)	5.16

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rrt2p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013A291 \| P38332 MIPS: YBR246W NCBI: 51012699 \| 536659 \| 586371 \| 6319723 \| NP_009805.1 GenBank/EMBL/DDBJ: DAA07362.1 \| AY692624 \| Z36115

Amino Acid Sequence (or in FASTA format)
1 MDSIQESDVL NAVKTKLPPC CLRIFRNKII LVGTYDLDKS TGYRSGSLDV 51 FTMDLKLLCS NNTYGAILDL KLSPFDDTLI CTAHSTGNIM LWRIRCTDKD 101 DFQSNELDIH AIANLQLFEK DVLIASCHFS PLDCKKLLVT NTAGEAATID 151 IRTLSVQFTA SAIAQAYSKL DKIDYEVQGA TEKVIHVESG QFLKPHELEC 201 WTAEFGSLQP FQDVVFTGGD DSRIMAHDLR SKEFIWSNNR IHDAGVVSIK 251 CSQPNFRNNK PTSIITGSYD DNIRSLDLRM MGESIFPGAN VPTVNKLACD 301 LGGGVWRFVE SPIDQEQSHH NGSDRLLVCC MYNGAKVVTM NDNSDEYFQI 351 QHYLKKGHDS MCYGGDWSNS LIATCSFYDN SLQTWIV*

Amino Acid Sequence (or in FASTA format)

       1  MDSIQESDVL NAVKTKLPPC CLRIFRNKII LVGTYDLDKS TGYRSGSLDV
      51  FTMDLKLLCS NNTYGAILDL KLSPFDDTLI CTAHSTGNIM LWRIRCTDKD
     101  DFQSNELDIH AIANLQLFEK DVLIASCHFS PLDCKKLLVT NTAGEAATID
     151  IRTLSVQFTA SAIAQAYSKL DKIDYEVQGA TEKVIHVESG QFLKPHELEC
     201  WTAEFGSLQP FQDVVFTGGD DSRIMAHDLR SKEFIWSNNR IHDAGVVSIK
     251  CSQPNFRNNK PTSIITGSYD DNIRSLDLRM MGESIFPGAN VPTVNKLACD
     301  LGGGVWRFVE SPIDQEQSHH NGSDRLLVCC MYNGAKVVTM NDNSDEYFQI
     351  QHYLKKGHDS MCYGGDWSNS LIATCSFYDN SLQTWIV*

external links for Rrt2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rrt2p

1)	Hontz RD, et al. (2009) Genetic Identification of Factors That Modulate Ribosomal DNA Transcription in Saccharomyces cerevisiae. Genetics 182(1):105-19
2)	Shi Y, et al. (2011) Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway. Mol Biol Cell 22(21):4093-107
3)	Su X, et al. (2012) Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc Natl Acad Sci U S A 109(49):19983-7
4)	Uthman S, et al. (2013) The Amidation Step of Diphthamide Biosynthesis in Yeast Requires DPH6, a Gene Identified through Mining the DPH1-DPH5 Interaction Network. PLoS Genet 9(2):e1003334
5)	Bonangelino CJ, et al. (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501
6)	Su X, et al. (2012) YBR246W is required for the third step of diphthamide biosynthesis. J Am Chem Soc 134(2):773-6
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41