THI2/YBR240C Protein Information

Standard Name	Thi2p 1
Systematic Name	Ybr240cp
Alias	Pho6p
ORF Classification	Verified
Description	Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type (1, 2, 3, 4, 5, 6) Also known as: phoF 7
Name Description	THIamine metabolism

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	450
Molecular Weight (Da)	51,822
Isoelectric Point (pI)	8.48

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Thi2p (InterPro)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136D79 \| P38141 MIPS: YBR240C NCBI: 2373393 \| 398365685 \| 536647 \| 586367 \| NP_009799.3 GenBank/EMBL/DDBJ: DAA07356.1 \| D32073 \| Z36109

Amino Acid Sequence (or in FASTA format)
1 MVNSKRQQRS KKVASSSKVP PTKGRTFTGC WACRFKKRRC DENRPICSLC 51 AKHGDNCSYD IRLMWLEENI YKVRKHSLIS SLQARKSKSK PLCQKISKSR 101 FKQMTHFRQL SPPTSDCEDS VHEASKETTL PNDNTFTISV RRLKIYNNAV 151 ASVFGSMTNR DYTQKRIDKK LDELLNMVEN DISVVNLNCS KHGPYSVFRA 201 NPAAVTSALT DQLPSPGHSM SSAEETTTAA LSSPPEDSTS LIDIIQGKIF 251 GILWFNCYGN MILNRQEYTT WFINKMRNSL TTEFIRFLGK IIDDPDINMA 301 SCLFKECIAR WSCVDWQSIA ITMLVIIHGY TCPNLTKLLR VWFLQQKLLR 351 FSMYPLVNFI INNTQDLDVL YHCNGLLGNA DLFEDPYQDE LTSELHVLVT 401 ERLVNSWKDT ILQQLCSCQD TTLSCSQLRY WQLQLKCNQQ FYKDVYAMQD 451 *

Amino Acid Sequence (or in FASTA format)

       1  MVNSKRQQRS KKVASSSKVP PTKGRTFTGC WACRFKKRRC DENRPICSLC
      51  AKHGDNCSYD IRLMWLEENI YKVRKHSLIS SLQARKSKSK PLCQKISKSR
     101  FKQMTHFRQL SPPTSDCEDS VHEASKETTL PNDNTFTISV RRLKIYNNAV
     151  ASVFGSMTNR DYTQKRIDKK LDELLNMVEN DISVVNLNCS KHGPYSVFRA
     201  NPAAVTSALT DQLPSPGHSM SSAEETTTAA LSSPPEDSTS LIDIIQGKIF
     251  GILWFNCYGN MILNRQEYTT WFINKMRNSL TTEFIRFLGK IIDDPDINMA
     301  SCLFKECIAR WSCVDWQSIA ITMLVIIHGY TCPNLTKLLR VWFLQQKLLR
     351  FSMYPLVNFI INNTQDLDVL YHCNGLLGNA DLFEDPYQDE LTSELHVLVT
     401  ERLVNSWKDT ILQQLCSCQD TTLSCSQLRY WQLQLKCNQQ FYKDVYAMQD
     451  *

external links for Thi2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Thi2p

1)	Nishimura H, et al. (1992) Cloning and characteristics of a positive regulatory gene, THI2 (PHO6), of thiamin biosynthesis in Saccharomyces cerevisiae. FEBS Lett 297(1-2):155-8
2)	Nosaka K, et al. (1994) Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae. J Biol Chem 269(48):30510-6
3)	Akache B, et al. (2001) Phenotypic analysis of genes encoding yeast zinc cluster proteins. Nucleic Acids Res 29(10):2181-90
4)	Nosaka K, et al. (2005) Genetic regulation mediated by thiamin pyrophosphate-binding motif in Saccharomyces cerevisiae. Mol Microbiol 58(2):467-79
5)	Mojzita D and Hohmann S (2006) Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae. Mol Genet Genomics 276(2):147-61
6)	Nosaka K, et al. (2008) Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae. FEBS Lett 582(29):3991-6
7)	Toh-e A, et al. (1975) Two new genes controlling the constitutive acid phosphatase synthesis in Saccharomyces cerevisiae. Mol Gen Genet 141(1):81-3