PCS60/YBR222C Protein Information

Standard Name	Pcs60p
Systematic Name	Ybr222cp
Alias	Fat2p
ORF Classification	Verified
Description	Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase (1, 2)

Experimental Data
Molecules/cell	8770 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	543
Molecular Weight (Da)	60,488
Isoelectric Point (pI)	9.98

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pcs60p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012A555 \| P38137 MIPS: YBR222C NCBI: 398365585 \| 536615 \| 586339 \| NP_009781.3 GenBank/EMBL/DDBJ: DAA07339.1 \| Z36091
External Classifications	EC: 6.-.-.- [Ligases]

Amino Acid Sequence (or in FASTA format)
1 MTSAATVTAS FNDTFSVSDN VAVIVPETDT QVTYRDLSHM VGHFQTMFTN 51 PNSPLYGAVF RQDTVAISMR NGLEFIVAFL GATMDAKIGA PLNPNYKEKE 101 FNFYLNDLKS KAICVPKGTT KLQSSEILKS ASTFGCFIVE LAFDATRFRV 151 EYDIYSPEDN YKRVIYRSLN NAKFVNTNPV KFPGFARSSD VALILHTSGT 201 TSTPKTVPLL HLNIVRSTLN IANTYKLTPL DRSYVVMPLF HVHGLIGVLL 251 STFRTQGSVV VPDGFHPKLF WDQFVKYNCN WFSCVPTISM IMLNMPKPNP 301 FPHIRFIRSC SSALAPATFH KLEKEFNAPV LEAYAMTEAS HQMTSNNLPP 351 GKRKPGTVGQ PQGVTVVILD DNDNVLPPGK VGEVSIRGEN VTLGYANNPK 401 ANKENFTKRE NYFRTGDQGY FDPEGFLVLT GRIKELINRG GEKISPIELD 451 GIMLSHPKID EAVAFGVPDD MYGQVVQAAI VLKKGEKMTY EELVNFLKKH 501 LASFKIPTKV YFVDKLPKTA TGKIQRRVIA ETFAKSSRNK SKL*

Amino Acid Sequence (or in FASTA format)

       1  MTSAATVTAS FNDTFSVSDN VAVIVPETDT QVTYRDLSHM VGHFQTMFTN
      51  PNSPLYGAVF RQDTVAISMR NGLEFIVAFL GATMDAKIGA PLNPNYKEKE
     101  FNFYLNDLKS KAICVPKGTT KLQSSEILKS ASTFGCFIVE LAFDATRFRV
     151  EYDIYSPEDN YKRVIYRSLN NAKFVNTNPV KFPGFARSSD VALILHTSGT
     201  TSTPKTVPLL HLNIVRSTLN IANTYKLTPL DRSYVVMPLF HVHGLIGVLL
     251  STFRTQGSVV VPDGFHPKLF WDQFVKYNCN WFSCVPTISM IMLNMPKPNP
     301  FPHIRFIRSC SSALAPATFH KLEKEFNAPV LEAYAMTEAS HQMTSNNLPP
     351  GKRKPGTVGQ PQGVTVVILD DNDNVLPPGK VGEVSIRGEN VTLGYANNPK
     401  ANKENFTKRE NYFRTGDQGY FDPEGFLVLT GRIKELINRG GEKISPIELD
     451  GIMLSHPKID EAVAFGVPDD MYGQVVQAAI VLKKGEKMTY EELVNFLKKH
     501  LASFKIPTKV YFVDKLPKTA TGKIQRRVIA ETFAKSSRNK SKL*

external links for Pcs60p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pcs60p

1)	Blobel F and Erdmann R (1996) Identification of a yeast peroxisomal member of the family of AMP-binding proteins. Eur J Biochem 240(2):468-76
2)	Tsvetanova NG, et al. (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41