PYC2/YBR218C Protein Information Help

Standard Name Pyc2p
Systematic Name Ybr218cp
ORF Classification Verified
Description Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description PYruvate Carboxylase
Experimental Data
Molecules/cell 17000 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,180
Molecular Weight (Da) 130,166
Isoelectric Point (pI) 6.47

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pyc2p (InterPro)
Physical Interactions There are 12 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000132BBF | P32327
MIPS: YBR218C
NCBI: 1041735 | 4255 | 536608 | 585765 | 6319695 | NP_009777.1
GenBank/EMBL/DDBJ: DAA07334.1 | U35647 | X59890 | Z36087
External Classifications EC: 6.4.1.1 [Pyruvate carboxylase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSSKKLAGL RDNFSLLGEK NKILVANRGE IPIRIFRSAH ELSMRTIAIY
      51  SHEDRLSMHR LKADEAYVIG EEGQYTPVGA YLAMDEIIEI AKKHKVDFIH
     101  PGYGFLSENS EFADKVVKAG ITWIGPPAEV IDSVGDKVSA RHLAARANVP
     151  TVPGTPGPIE TVQEALDFVN EYGYPVIIKA AFGGGGRGMR VVREGDDVAD
     201  AFQRATSEAR TAFGNGTCFV ERFLDKPKHI EVQLLADNHG NVVHLFERDC
     251  SVQRRHQKVV EVAPAKTLPR EVRDAILTDA VKLAKVCGYR NAGTAEFLVD
     301  NQNRHYFIEI NPRIQVEHTI TEEITGIDIV SAQIQIAAGA TLTQLGLLQD
     351  KITTRGFSIQ CRITTEDPSK NFQPDTGRLE VYRSAGGNGV RLDGGNAYAG
     401  ATISPHYDSM LVKCSCSGST YEIVRRKMIR ALIEFRIRGV KTNIPFLLTL
     451  LTNPVFIEGT YWTTFIDDTP QLFQMVSSQN RAQKLLHYLA DLAVNGSSIK
     501  GQIGLPKLKS NPSVPHLHDA QGNVINVTKS APPSGWRQVL LEKGPSEFAK
     551  QVRQFNGTLL MDTTWRDAHQ SLLATRVRTH DLATIAPTTA HALAGAFALE
     601  CWGGATFDVA MRFLHEDPWE RLRKLRSLVP NIPFQMLLRG ANGVAYSSLP
     651  DNAIDHFVKQ AKDNGVDIFR VFDALNDLEQ LKVGVNAVKK AGGVVEATVC
     701  YSGDMLQPGK KYNLDYYLEV VEKIVQMGTH ILGIKDMAGT MKPAAAKLLI
     751  GSLRTRYPDL PIHVHSHDSA GTAVASMTAC ALAGADVVDV AINSMSGLTS
     801  QPSINALLAS LEGNIDTGIN VEHVRELDAY WAEMRLLYSC FEADLKGPDP
     851  EVYQHEIPGG QLTNLLFQAQ QLGLGEQWAE TKRAYREANY LLGDIVKVTP
     901  TSKVVGDLAQ FMVSNKLTSD DIRRLANSLD FPDSVMDFFE GLIGQPYGGF
     951  PEPLRSDVLR NKRRKLTCRP GLELEPFDLE KIREDLQNRF GDIDECDVAS
    1001  YNMYPRVYED FQKIRETYGD LSVLPTKNFL APAEPDEEIE VTIEQGKTLI
    1051  IKLQAVGDLN KKTGQREVYF ELNGELRKIR VADKSQNIQS VAKPKADVHD
    1101  THQIGAPMAG VIIEVKVHKG SLVKKGESIA VLSAMKMEMV VSSPADGQVK
    1151  DVFIKDGESV DASDLLVVLE EETLPPSQKK *

external links for Pyc2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Pyc2p
1) Walker ME, et al.  (1991) Yeast pyruvate carboxylase: identification of two genes encoding isoenzymes. Biochem Biophys Res Commun 176(3):1210-7
2) Brewster NK, et al.  (1994) Regulation of pyruvate carboxylase isozyme (PYC1, PYC2) gene expression in Saccharomyces cerevisiae during fermentative and nonfermentative growth. Arch Biochem Biophys 311(1):62-71
3) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41