ATG12/YBR217W Protein Information

Standard Name	Atg12p 1
Systematic Name	Ybr217wp
Alias	Apg12p 2
ORF Classification	Verified
Description	Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation (2, 3, 4, 5, 6, 7)
Name Description	AuTophaGy related 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	186
Molecular Weight (Da)	21,106
Isoelectric Point (pI)	6.77

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Atg12p (InterPro)
Physical Interactions	There are 78 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125C67 \| P38316 MIPS: YBR217W NCBI: 3688623 \| 433286619 \| 45269303 \| 536606 \| 586336 \| 6319694 \| NP_009776.1 GenBank/EMBL/DDBJ: DAA07333.1 \| AB017924 \| AY557706 \| Z36086

Amino Acid Sequence (or in FASTA format)
1 MSRILESENE TESDESSIIS TNNGTAMERS RNNQELRSSP HTVQNRLELF 51 SRRLSQLGLA SDISVDQQVE DSSSGTYEQE ETIKTNAQTS KQKSHKDEKN 101 IQKIQIKFQP IGSIGQLKPS VCKISMSQSF AMVILFLKRR LKMDHVYCYI 151 NNSFAPSPQQ NIGELWMQFK TNDELIVSYC ASVAFG*

Amino Acid Sequence (or in FASTA format)

       1  MSRILESENE TESDESSIIS TNNGTAMERS RNNQELRSSP HTVQNRLELF
      51  SRRLSQLGLA SDISVDQQVE DSSSGTYEQE ETIKTNAQTS KQKSHKDEKN
     101  IQKIQIKFQP IGSIGQLKPS VCKISMSQSF AMVILFLKRR LKMDHVYCYI
     151  NNSFAPSPQQ NIGELWMQFK TNDELIVSYC ASVAFG*

external links for Atg12p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Atg12p

1)	Klionsky DJ, et al. (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45
2)	Tsukada M and Ohsumi Y (1993) Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 333(1-2):169-74
3)	Mizushima N, et al. (1998) A protein conjugation system essential for autophagy. Nature 395(6700):395-8
4)	Shintani T, et al. (1999) Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. EMBO J 18(19):5234-41
5)	Tanida I, et al. (1999) Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy. Mol Biol Cell 10(5):1367-79
6)	Kuma A, et al. (2002) Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem 277(21):18619-25
7)	Hanada T, et al. (2007) The Atg12-Atg5 Conjugate Has a Novel E3-like Activity for Protein Lipidation in Autophagy. J Biol Chem 282(52):37298-302