MET8/YBR213W Protein Information

Standard Name	Met8p 1, 2
Systematic Name	Ybr213wp
ORF Classification	Verified
Description	Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis (3, 4, 5)
Name Description	METhionine requiring 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	274
Molecular Weight (Da)	31,917
Isoelectric Point (pI)	6.26

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Met8p (InterPro)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012EF4E \| P15807 MIPS: YBR213W NCBI: 127028 \| 21465956 \| 21465957 \| 21465958 \| 3934 \| 536598 \| 6319690 \| NP_009772.1 GenBank/EMBL/DDBJ: DAA07329.1 \| X17271 \| Z36082
External Classifications	EC: 1.3.1.76 [Precorrin-2 dehydrogenase] EC: 4.99.1.4 [Sirohydrochlorin ferrochelatase]

Amino Acid Sequence (or in FASTA format)
1 MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI 51 IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL 101 EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF 151 GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL 201 GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV 251 MFQEQNCSLQ FPPRERLLSE YCSS*

Amino Acid Sequence (or in FASTA format)

       1  MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI
      51  IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL
     101  EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF
     151  GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL
     201  GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV
     251  MFQEQNCSLQ FPPRERLLSE YCSS*

external links for Met8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Met8p

1)	Masselot M and De Robichon-Szulmajster H (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2)	Nelbock, P., et al. (1989) ; Personal Communication, Mortimer Map Edition 10
3)	Raux E, et al. (1999) The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis. Biochem J 338 ( Pt 3)():701-8
4)	Thomas D, et al. (1992) Physiological analysis of mutants of Saccharomyces cerevisiae impaired in sulphate assimilation. J Gen Microbiol 138(10):2021-8
5)	Schubert HL, et al. (2002) The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase. EMBO J 21(9):2068-75