DUR1,2/YBR208C Protein Information

Standard Name	Dur1,2p 1
Systematic Name	Ybr208cp
Alias	Dur80p
ORF Classification	Verified
Description	Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description	Degradation of URea 1

Experimental Data
Molecules/cell	952 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,835
Molecular Weight (Da)	201,830
Isoelectric Point (pI)	5.39

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dur1,2p (InterPro)
Physical Interactions	There are 13 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FB4 \| P32528 MIPS: YBR208C NCBI: 173122 \| 311684 \| 536588 \| 585072 \| 6319685 \| NP_009767.1 GenBank/EMBL/DDBJ: DAA07325.1 \| M64926 \| Z21487 \| Z36077
External Classifications	EC: 3.5.1.54 [Allophanate hydrolase] EC: 6.3.4.6 [Urea carboxylase]

Amino Acid Sequence (or in FASTA format)
1 MTVSSDTTAE ISLGWSIQDW IDFHKSSSSQ ASLRLLESLL DSQNVAPVDN 51 AWISLISKEN LLHQFQILKS RENKETLPLY GVPIAVKDNI DVRGLPTTAA 101 CPSFAYEPSK DSKVVELLRN AGAIIVGKTN LDQFATGLVG TRSPYGKTPC 151 AFSKEHVSGG SSAGSASVVA RGIVPIALGT DTAGSGRVPA ALNNLIGLKP 201 TKGVFSCQGV VPACKSLDCV SIFALNLSDA ERCFRIMCQP DPDNDEYSRP 251 YVSNPLKKFS SNVTIAIPKN IPWYGETKNP VLFSNAVENL SRTGANVIEI 301 DFEPLLELAR CLYEGTWVAE RYQAIQSFLD SKPPKESLDP TVISIIEGAK 351 KYSAVDCFSF EYKRQGILQK VRRLLESVDV LCVPTCPLNP TMQQVADEPV 401 LVNSRQGTWT NFVNLADLAA LAVPAGFRDD GLPNGITLIG KKFTDYALLE 451 LANRYFQNIF PNGSRTYGTF TSSSVKPAND QLVGPDYDPS TSIKLAVVGA 501 HLKGLPLHWQ LEKVNATYLC TTKTSKAYQL FALPKNGPVL KPGLRRVQDS 551 NGSQIELEVY SVPKELFGAF ISMVPEPLGI GSVELESGEW IKSFICEESG 601 YKAKGTVDIT KYGGFRAYFE MLKKKESQKK KLFDTVLIAN RGEIAVRIIK 651 TLKKLGIRSV AVYSDPDKYS QHVTDADVSV PLHGTTAAQT YLDMNKIIDA 701 AKQTNAQAII PGYGFLSENA DFSDACTSAG ITFVGPSGDI IRGLGLKHSA 751 RQIAQKAGVP LVPGSLLITS VEEAKKVAAE LEYPVMVKST AGGGGIGLQK 801 VDSEEDIEHI FETVKHQGET FFGDAGVFLE RFIENARHVE VQLMGDGFGK 851 AIALGERDCS LQRRNQKVIE ETPAPNLPEK TRLALRKAAE SLGSLLNYKC 901 AGTVEFIYDE KKDEFYFLEV NTRLQVEHPI TEMVTGLDLV EWMIRIAAND 951 APDFDSTKVE VNGVSMEARL YAENPLKNFR PSPGLLVDVK FPDWARVDTW 1001 VKKGTNISPE YDPTLAKIIV HGKDRDDAIS KLNQALEETK VYGCITNIDY 1051 LKSIITSDFF AKAKVSTNIL NSYQYEPTAI EITLPGAHTS IQDYPGRVGY 1101 WRIGVPPSGP MDAYSFRLAN RIVGNDYRTP AIEVTLTGPS IVFHCETVIA 1151 ITGGTALCTL DGQEIPQHKP VEVKRGSTLS IGKLTSGCRA YLGIRGGIDV 1201 PKYLGSYSTF TLGNVGGYNG RVLKLGDVLF LPSNEENKSV ECLPQNIPQS 1251 LIPQISETKE WRIGVTCGPH GSPDFFKPES IEEFFSEKWK VHYNSNRFGV 1301 RLIGPKPKWA RSNGGEGGMH PSNTHDYVYS LGAINFTGDE PVIITCDGPS 1351 LGGFVCQAVV PEAELWKVGQ VKPGDSIQFV PLSYESSRSL KESQDVAIKS 1401 LDGTKLRRLD SVSILPSFET PILAQMEKVN ELSPKVVYRQ AGDRYVLVEY 1451 GDNEMNFNIS YRIECLISLV KKNKTIGIVE MSQGVRSVLI EFDGYKVTQK 1501 ELLKVLVAYE TEIQFDENWK ITSNIIRLPM AFEDSKTLAC VQRYQETIRS 1551 SAPWLPNNVD FIANVNGISR NEVYDMLYSA RFMVLGLGDV FLGSPCAVPL 1601 DPRHRFLGSK YNPSRTYTER GAVGIGGMYM CIYAANSPGG YQLVGRTIPI 1651 WDKLCLAASS EVPWLMNPFD QVEFYPVSEE DLDKMTEDCD NGVYKVNIEK 1701 SVFDHQEYLR WINANKDSIT AFQEGQLGER AEEFAKLIQN ANSELKESVT 1751 VKPDEEEDFP EGAEIVYSEY SGRFWKSIAS VGDVIEAGQG LLIIEAMKAE 1801 MIISAPKSGK IIKICHGNGD MVDSGDIVAV IETLA*

Amino Acid Sequence (or in FASTA format)

       1  MTVSSDTTAE ISLGWSIQDW IDFHKSSSSQ ASLRLLESLL DSQNVAPVDN
      51  AWISLISKEN LLHQFQILKS RENKETLPLY GVPIAVKDNI DVRGLPTTAA
     101  CPSFAYEPSK DSKVVELLRN AGAIIVGKTN LDQFATGLVG TRSPYGKTPC
     151  AFSKEHVSGG SSAGSASVVA RGIVPIALGT DTAGSGRVPA ALNNLIGLKP
     201  TKGVFSCQGV VPACKSLDCV SIFALNLSDA ERCFRIMCQP DPDNDEYSRP
     251  YVSNPLKKFS SNVTIAIPKN IPWYGETKNP VLFSNAVENL SRTGANVIEI
     301  DFEPLLELAR CLYEGTWVAE RYQAIQSFLD SKPPKESLDP TVISIIEGAK
     351  KYSAVDCFSF EYKRQGILQK VRRLLESVDV LCVPTCPLNP TMQQVADEPV
     401  LVNSRQGTWT NFVNLADLAA LAVPAGFRDD GLPNGITLIG KKFTDYALLE
     451  LANRYFQNIF PNGSRTYGTF TSSSVKPAND QLVGPDYDPS TSIKLAVVGA
     501  HLKGLPLHWQ LEKVNATYLC TTKTSKAYQL FALPKNGPVL KPGLRRVQDS
     551  NGSQIELEVY SVPKELFGAF ISMVPEPLGI GSVELESGEW IKSFICEESG
     601  YKAKGTVDIT KYGGFRAYFE MLKKKESQKK KLFDTVLIAN RGEIAVRIIK
     651  TLKKLGIRSV AVYSDPDKYS QHVTDADVSV PLHGTTAAQT YLDMNKIIDA
     701  AKQTNAQAII PGYGFLSENA DFSDACTSAG ITFVGPSGDI IRGLGLKHSA
     751  RQIAQKAGVP LVPGSLLITS VEEAKKVAAE LEYPVMVKST AGGGGIGLQK
     801  VDSEEDIEHI FETVKHQGET FFGDAGVFLE RFIENARHVE VQLMGDGFGK
     851  AIALGERDCS LQRRNQKVIE ETPAPNLPEK TRLALRKAAE SLGSLLNYKC
     901  AGTVEFIYDE KKDEFYFLEV NTRLQVEHPI TEMVTGLDLV EWMIRIAAND
     951  APDFDSTKVE VNGVSMEARL YAENPLKNFR PSPGLLVDVK FPDWARVDTW
    1001  VKKGTNISPE YDPTLAKIIV HGKDRDDAIS KLNQALEETK VYGCITNIDY
    1051  LKSIITSDFF AKAKVSTNIL NSYQYEPTAI EITLPGAHTS IQDYPGRVGY
    1101  WRIGVPPSGP MDAYSFRLAN RIVGNDYRTP AIEVTLTGPS IVFHCETVIA
    1151  ITGGTALCTL DGQEIPQHKP VEVKRGSTLS IGKLTSGCRA YLGIRGGIDV
    1201  PKYLGSYSTF TLGNVGGYNG RVLKLGDVLF LPSNEENKSV ECLPQNIPQS
    1251  LIPQISETKE WRIGVTCGPH GSPDFFKPES IEEFFSEKWK VHYNSNRFGV
    1301  RLIGPKPKWA RSNGGEGGMH PSNTHDYVYS LGAINFTGDE PVIITCDGPS
    1351  LGGFVCQAVV PEAELWKVGQ VKPGDSIQFV PLSYESSRSL KESQDVAIKS
    1401  LDGTKLRRLD SVSILPSFET PILAQMEKVN ELSPKVVYRQ AGDRYVLVEY
    1451  GDNEMNFNIS YRIECLISLV KKNKTIGIVE MSQGVRSVLI EFDGYKVTQK
    1501  ELLKVLVAYE TEIQFDENWK ITSNIIRLPM AFEDSKTLAC VQRYQETIRS
    1551  SAPWLPNNVD FIANVNGISR NEVYDMLYSA RFMVLGLGDV FLGSPCAVPL
    1601  DPRHRFLGSK YNPSRTYTER GAVGIGGMYM CIYAANSPGG YQLVGRTIPI
    1651  WDKLCLAASS EVPWLMNPFD QVEFYPVSEE DLDKMTEDCD NGVYKVNIEK
    1701  SVFDHQEYLR WINANKDSIT AFQEGQLGER AEEFAKLIQN ANSELKESVT
    1751  VKPDEEEDFP EGAEIVYSEY SGRFWKSIAS VGDVIEAGQG LLIIEAMKAE
    1801  MIISAPKSGK IIKICHGNGD MVDSGDIVAV IETLA*

external links for Dur1,2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Dur1,2p

1)	Cooper TG, et al. (1980) Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. Genetics 94(3):555-80
2)	van Vuuren HJ, et al. (1991) Upstream induction sequence, the cis-acting element required for response to the allantoin pathway inducer and enhancement of operation of the nitrogen-regulated upstream activation sequence in Saccharomyces cerevisiae. J Bacteriol 173(22):7186-95
3)	Cox KH, et al. (2000) Saccharomyces cerevisiae GATA sequences function as TATA elements during nitrogen catabolite repression and when Gln3p is excluded from the nucleus by overproduction of Ure2p. J Biol Chem 275(23):17611-8
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41