MSI1/YBR195C Protein Information

Standard Name	Msi1p 1
Systematic Name	Ybr195cp
Alias	Cac3p
ORF Classification	Verified
Description	Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase (10, 11, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description	Multicopy Suppressor of IRA1 12

Experimental Data
Molecules/cell	149 13

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	422
Molecular Weight (Da)	47,364
Isoelectric Point (pI)	5.42

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Msi1p (InterPro)
Physical Interactions	There are 32 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012F60D \| P13712 MIPS: YBR195C NCBI: 127346 \| 172006 \| 311671 \| 51013119 \| 536563 \| 6319672 \| NP_009754.1 GenBank/EMBL/DDBJ: DAA07309.1 \| AY692834 \| M27300 \| Z21487 \| Z36064

Amino Acid Sequence (or in FASTA format)
1 MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ 51 FFPDLDTTSD EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD 101 MDEMEFKPEN STRFPSKHLV NDISIFFPNG ECNRARYLPQ NPDIIAGASS 151 DGAIYIFDRT KHGSTRIRQS KISHPFETKL FGSHGVIQDV EAMDTSSADI 201 NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII DLPLVSINSD 251 GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN 301 SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP 351 NFDTVLATAG QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM 401 CSVANDNSVH IWKPAGNLVG HS*

Amino Acid Sequence (or in FASTA format)

       1  MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ
      51  FFPDLDTTSD EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD
     101  MDEMEFKPEN STRFPSKHLV NDISIFFPNG ECNRARYLPQ NPDIIAGASS
     151  DGAIYIFDRT KHGSTRIRQS KISHPFETKL FGSHGVIQDV EAMDTSSADI
     201  NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII DLPLVSINSD
     251  GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN
     301  SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP
     351  NFDTVLATAG QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM
     401  CSVANDNSVH IWKPAGNLVG HS*

external links for Msi1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Msi1p

1)	Spevak, W. (1992) Personal Communication, Mortimer Map Edition 11
2)	Enomoto S and Berman J (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
3)	Kaufman PD, et al. (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
4)	Game JC and Kaufman PD (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
5)	Smith JS, et al. (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
6)	Johnston SD, et al. (2001) CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1. Mol Cell Biol 21(5):1784-94
7)	Sharp JA, et al. (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
8)	Zabaronick SR and Tyler JK (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
9)	Zhou H, et al. (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
10)	Kim JA and Haber JE (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
11)	Kim HJ, et al. (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31
12)	Ruggieri R, et al. (1989) MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 86(22):8778-82
13)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41