MED8/YBR193C Protein Information

Standard Name	Med8p 1
Systematic Name	Ybr193cp
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description	MEDiator complex 1

Experimental Data
Molecules/cell	937 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	223
Molecular Weight (Da)	25,268
Isoelectric Point (pI)	5.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Med8p (InterPro)
Physical Interactions	There are 144 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053155 \| P38304 MIPS: YBR193C NCBI: 116667917 \| 116667918 \| 116667919 \| 116667920 \| 311669 \| 345100886 \| 345100893 \| 345100900 \| 398365395 \| 410563051 \| 410563058 \| 45269313 \| 459358308 \| 536559 \| 586321 \| NP_009752.3 GenBank/EMBL/DDBJ: DAA07307.1 \| AY557711 \| Z21487 \| Z36062

Amino Acid Sequence (or in FASTA format)
1 MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI 51 RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK 101 FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ 151 QDREITNWAR TTFRNEYGKH DFKNEESLSE EHASLLVRDS KPSKPFNVDD 201 VLKFTFTGEK PIITGSTSTS SSN*

Amino Acid Sequence (or in FASTA format)

       1  MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI
      51  RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK
     101  FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ
     151  QDREITNWAR TTFRNEYGKH DFKNEESLSE EHASLLVRDS KPSKPFNVDD
     201  VLKFTFTGEK PIITGSTSTS SSN*

external links for Med8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Med8p

1)	Myers LC, et al. (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41