PCH2/YBR186W Protein Information

Standard Name	Pch2p 1
Systematic Name	Ybr186wp
ORF Classification	Verified
Description	Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation (1, 2, 3)
Name Description	Pachytene CHeckpoint 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	564
Molecular Weight (Da)	63,460
Isoelectric Point (pI)	5.76

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pch2p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001682C2 \| P38126 MIPS: YBR186W NCBI: 114152857 \| 536546 \| 575913 \| 586317 \| 9755327 \| NP_009745.2 GenBank/EMBL/DDBJ: DAA07301.1 \| U02073 \| Z36055

Amino Acid Sequence (or in FASTA format)
1 MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL 51 LKAVVKRKLE SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR 101 SLAKVLLHQF SSINGKVNTV NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL 151 NFHEKINLGQ HIDSILDSEE TNESDTYHMG SVDEFIIYPF CCLEEQDELK 201 NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP LSKQTLNLVN 251 ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI 301 TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG 351 IIIELSCARI FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV 401 EAIASSRTNL SSRNESTDGI RVVNTLLTQL DRLKKYHNFL ALATSNLLDS 451 LDDAFVDRAD GVFYVGNPTA EGILHILKVC IEEMITSGII LFHARSTGVK 501 FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT FPVDDDEFVL 551 ALAMSARKLS AARK*

Amino Acid Sequence (or in FASTA format)

       1  MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL
      51  LKAVVKRKLE SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR
     101  SLAKVLLHQF SSINGKVNTV NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL
     151  NFHEKINLGQ HIDSILDSEE TNESDTYHMG SVDEFIIYPF CCLEEQDELK
     201  NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP LSKQTLNLVN
     251  ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI
     301  TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG
     351  IIIELSCARI FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV
     401  EAIASSRTNL SSRNESTDGI RVVNTLLTQL DRLKKYHNFL ALATSNLLDS
     451  LDDAFVDRAD GVFYVGNPTA EGILHILKVC IEEMITSGII LFHARSTGVK
     501  FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT FPVDDDEFVL
     551  ALAMSARKLS AARK*

external links for Pch2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Pch2p

1)	San-Segundo PA and Roeder GS (1999) Pch2 links chromatin silencing to meiotic checkpoint control. Cell 97(3):313-24
2)	San-Segundo PA and Roeder GS (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3)	Farmer S, et al. (2012) Budding yeast pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS One 7(6):e39724