FZO1/YBR179C Protein Information

Standard Name	Fzo1p 1
Systematic Name	Ybr179cp
ORF Classification	Verified
Description	Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system (1, 2, 3, 4, 5, 6)
Name Description	FuZzy Onions homolog 7

Experimental Data
Molecules/cell	1010 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	855
Molecular Weight (Da)	97,807
Isoelectric Point (pI)	6.99

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fzo1p (InterPro)
Physical Interactions	There are 25 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013A1FA \| P38297 MIPS: YBR179C NCBI: 536530 \| 586311 \| 6319656 \| NP_009738.1 \| NM_001178527.1 GenBank/EMBL/DDBJ: DAA07293.1 \| Z36048
External Classifications	EC: 3.6.5.- [Hydrolases, Acting on GTP; involved in cellular and subcellular movement]

Amino Acid Sequence (or in FASTA format)
1 MSEGKQQFKD SNKPHKDSTD QDDDAATIVP QTLTYSRNEG HFLGSNFHGV 51 TDDRTTLFDG EEGRREDDLL PSLRSSNSKA HLISSQLSQW NYNNNRVLLK 101 RSILKTQAFM DQLQEENNIR PIFIAANDER EKLHVLQLNI KLDGQYNTKE 151 KNGFNIEKKA LSKLFHSQIV SVTNHLNALK KRVDDVSSKV FITGDVNTGK 201 SALCNSLLKQ RLLPEDQLPC TNVFSEILEA RENDGIEEVH AIPLNIAPTL 251 KEAIDMYSIQ NPKTYEIHTL KELPDLVPQN GKYALLKIYI KDDKRPASTS 301 LLRNGTVDIS LIDSPGLNMD SLQTAEVMSR QEEIDLVIFV VNAENQLTLS 351 AKEFISLASR EKKLMFFVVK KFDKIRDKQR CKELILKQIR DLSPETYKRA 401 ADFVHFVSKN GDELPHYHNE NDNEDHGDRK PDDDPYSSSD PDPDFDSLED 451 SLRNFVLKKR SLSKLLPAKT YLSKLLSDII MISKSNMKMY SEEEIKINEQ 501 LETLRPEILS ARAKCNDLTT SVDQMAEQTI TMTYNNTKEA LLNALDVPLH 551 EYPKYQGLGQ IYDFIFSTEA FIANQIDESI GSSELFAKQK TDLLVKKIYE 601 IGKNELGDDF MCERVFRSEL MFRKRKHLIG KRLKVSLSIT DLFAPTWKGF 651 LSYLSWQKPV TAPLPDIEGQ TNEGQIGLMK YLGLKNYPLT QYWSRPSLLF 701 TSKIPTLTLY FLGSTKVVGN IILNGIKLSS WSSLKKLSVP VIVVGSLLGL 751 TYLIHDLPRA LPMNLSIKYK RKLQELDYIH LNAQRTSNEV RDVLRVPTRE 801 ILRSCEIIMD KKQITKKELE NKKESNLLSI KFFQSLYEGT VAQKLMVEEI 851 NLDID*

Amino Acid Sequence (or in FASTA format)

       1  MSEGKQQFKD SNKPHKDSTD QDDDAATIVP QTLTYSRNEG HFLGSNFHGV
      51  TDDRTTLFDG EEGRREDDLL PSLRSSNSKA HLISSQLSQW NYNNNRVLLK
     101  RSILKTQAFM DQLQEENNIR PIFIAANDER EKLHVLQLNI KLDGQYNTKE
     151  KNGFNIEKKA LSKLFHSQIV SVTNHLNALK KRVDDVSSKV FITGDVNTGK
     201  SALCNSLLKQ RLLPEDQLPC TNVFSEILEA RENDGIEEVH AIPLNIAPTL
     251  KEAIDMYSIQ NPKTYEIHTL KELPDLVPQN GKYALLKIYI KDDKRPASTS
     301  LLRNGTVDIS LIDSPGLNMD SLQTAEVMSR QEEIDLVIFV VNAENQLTLS
     351  AKEFISLASR EKKLMFFVVK KFDKIRDKQR CKELILKQIR DLSPETYKRA
     401  ADFVHFVSKN GDELPHYHNE NDNEDHGDRK PDDDPYSSSD PDPDFDSLED
     451  SLRNFVLKKR SLSKLLPAKT YLSKLLSDII MISKSNMKMY SEEEIKINEQ
     501  LETLRPEILS ARAKCNDLTT SVDQMAEQTI TMTYNNTKEA LLNALDVPLH
     551  EYPKYQGLGQ IYDFIFSTEA FIANQIDESI GSSELFAKQK TDLLVKKIYE
     601  IGKNELGDDF MCERVFRSEL MFRKRKHLIG KRLKVSLSIT DLFAPTWKGF
     651  LSYLSWQKPV TAPLPDIEGQ TNEGQIGLMK YLGLKNYPLT QYWSRPSLLF
     701  TSKIPTLTLY FLGSTKVVGN IILNGIKLSS WSSLKKLSVP VIVVGSLLGL
     751  TYLIHDLPRA LPMNLSIKYK RKLQELDYIH LNAQRTSNEV RDVLRVPTRE
     801  ILRSCEIIMD KKQITKKELE NKKESNLLSI KFFQSLYEGT VAQKLMVEEI
     851  NLDID*

external links for Fzo1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Fzo1p

1)	Rapaport D, et al. (1998) Fzo1p is a mitochondrial outer membrane protein essential for the biogenesis of functional mitochondria in Saccharomyces cerevisiae. J Biol Chem 273(32):20150-5
2)	Hermann GJ, et al. (1998) Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. J Cell Biol 143(2):359-73
3)	Escobar-Henriques M, et al. (2006) Regulation of mitochondrial fusion by the F-box protein Mdm30 involves proteasome-independent turnover of Fzo1. J Cell Biol 173(5):645-50
4)	Cohen MM, et al. (2008) Ubiquitin-Proteasome-dependent Degradation of a Mitofusin, a Critical Regulator of Mitochondrial Fusion. Mol Biol Cell 19(6):2457-64
5)	Anton F, et al. (2011) Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion. J Cell Sci 124(Pt 7):1126-35
6)	Cohen MM, et al. (2011) Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion. J Cell Sci 124(Pt 9):1403-10
7)	Hales KG and Fuller MT (1997) Developmentally regulated mitochondrial fusion mediated by a conserved, novel, predicted GTPase. Cell 90(1):121-9
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41