SMY2/YBR172C Protein Information Help

Standard Name Smy2p 1
Systematic Name Ybr172cp
ORF Classification Verified
Description Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description Suppressor of MYo2-66 1
Experimental Data
Molecules/cell 486 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 740
Molecular Weight (Da) 81,396
Isoelectric Point (pI) 9.54

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Smy2p (InterPro)
Physical Interactions There are 28 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001D4767 | P32909
MIPS: YBR172C
NCBI: 110283001 | 172628 | 281500733 | 281500734 | 281500735 | 281500736 | 281500737 | 296278439 | 398365269 | 402610 | 417787 | 536518 | NP_009731.4
GenBank/EMBL/DDBJ: DAA07288.1 | M90654 | X74437 | Z36041
Amino Acid Sequence (or in FASTA format)
 
       1  MIAPDSQRLF GSFDEQFKDL KLDSVDTENN NTHGVSTILD SSPASVNNNT
      51  NGAVAASVNT VPGSTFRSNT PLLGGRHPLS RTSSLIDSIG IQRAASPFSS
     101  MKEPFIPQSS GVMSSSFWHG DHPESRVSTP VQQHPLLQRN ESSSSFSYAA
     151  NLGVNLSTHS LAVDITPLST PTAAQSHVNL FPSSDIPPNM SMNGMSQLPA
     201  PVSVESSWRY IDTQGQIHGP FTTQMMSQWY IGGYFASTLQ ISRLGSTPET
     251  LGINDIFITL GELMTKLEKY DTDPFTTFDK LHVQTTSSDS INLNLAPYAS
     301  GVAATGTIKA TENDIFKPLT HDNIWDMDGG TTSKGVDIKL ASATTISQTD
     351  ESHKQEYKST TMLEKGKKEK SESVAKALLD EQEKRNRELK RKEEARLSKK
     401  QKQKEDDLLK KQKEQKEQKE KEALEAEKQK KSEKTKKDTQ TQTEGFKTSK
     451  DLPSLNSSSA NPAPWASKVK VNNAIETSIK NGVSSTGKKK GEPLGLQQRN
     501  SKEEKQKEEL KSVLNWANKS SLPSNQTIDI KSQFQKSPKG MKESSPLKEL
     551  EDPNFIEEQK KLWEKVQSSS KQVKSTSSAS TTTSSWTTVT SKGKAPIGTV
     601  VSPYSKTNTS LNSSLTAKTS TTSTTTTFAS MNNVSPRQEF IKWCKSQMKL
     651  NSGITNNNVL ELLLSLPTGP ESKELIQETI YANSDVMDGR RFATEFIKRR
     701  VACEKQGDDP LSWNEALALS GNDDDGWEFQ VVSKKKGRKH *

external links for Smy2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Smy2p
1) Lillie SH and Brown SS  (1994) Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae. J Cell Biol 125(4):825-42
2) Andoh T, et al.  (2004) The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase. Biochem Biophys Res Commun 317(4):1138-43
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Higashio H, et al.  (2008) Smy2p Participates in COPII Vesicle Formation Through the Interaction with Sec23p/Sec24p Subcomplex. Traffic 9(1):79-93
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41