CDC28/YBR160W Protein Information Help

Standard Name Cdc28p 1, 2
Systematic Name Ybr160wp
Alias Cdk1p , Hsl5p , Srm5p
ORF Classification Verified
Description Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1 (CLNs), S and G2/M (CLBs) phase cyclins, which provide substrate specificity; regulates cell cycle and basal transcription, chromosome duplication and segregation, lipid biosynthesis, membrane trafficking, polarized growth, and morphogenesis; abundance increases in DNA replication stress; transcript induction in osmostress involves antisense RNA (10, 11, 12, 13, 14, 15, 3, 4, 5, 6, 7, 8, 9)
Name Description Cell Division Cycle 16
Experimental Data
Molecules/cell 6670 17
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 298
Molecular Weight (Da) 34,061
Isoelectric Point (pI) 8.5

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cdc28p (InterPro)
Physical Interactions There are 640 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F53 | P00546
MIPS: YBR160W
NCBI: 115915 | 3486 | 398365175 | 535469 | 536494 | NP_009718.3 | NM_001178508.3
GenBank/EMBL/DDBJ: CAA25065.1 | CAA56509.1 | CAA85119.1 | DAA07275.1 | X00257 | X80224 | Z36029
External Classifications EC: 2.7.11.22 [Cyclin-dependent kinase]
Amino Acid Sequence (or in FASTA format)
       1  MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG
      51  VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME
     101  GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL
     151  KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC
     201  IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP
     251  QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES* 

external links for Cdc28p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains YeastRC Public Image Repository
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cdc28p
1) Nasmyth KA and Reed SI  (1980) Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci U S A 77(4):2119-23
2) Hartwell LH, et al.  (1973) Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics 74(2):267-286
3) Reed SI  (1980) The selection of S. cerevisiae mutants defective in the start event of cell division. Genetics 95(3):561-77
4) Mendenhall MD and Hodge AE  (1998) Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae. Microbiol Mol Biol Rev 62(4):1191-243
5) Reed SI and Wittenberg C  (1990) Mitotic role for the Cdc28 protein kinase of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 87(15):5697-701
6) Wittenberg C, et al.  (1990) G1-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase. Cell 62(2):225-37
7) Purnapatre K, et al.  (2002) The CLN3/SWI6/CLN2 pathway and SNF1 act sequentially to regulate meiotic initiation in Saccharomyces cerevisiae. Genes Cells 7(7):675-91
8) Benjamin KR, et al.  (2003) Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2. Genes Dev 17(12):1524-39
9) Hartwell LH  (1973) Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol 115(3):966-74
10) Kurat CF, et al.  (2009) Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links lipolysis to cell-cycle progression. Mol Cell 33(1):53-63
11) Enserink JM and Kolodner RD  (2010) An overview of Cdk1-controlled targets and processes. Cell Div 5():11
12) Chymkowitch P, et al.  (2012) Cdc28 kinase activity regulates the basal transcription machinery at a subset of genes. Proc Natl Acad Sci U S A 109(26):10450-5
13) McCusker D, et al.  (2012) Cdk1-dependent control of membrane-trafficking dynamics. Mol Biol Cell 23(17):3336-47
14) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
15) Nadal-Ribelles M, et al.  (2014) Control of Cdc28 CDK1 by a Stress-Induced lncRNA. Mol Cell 53(4):549-61
16) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
17) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41