SUP45/YBR143C Protein Information Help

Standard Name Sup45p
Systematic Name Ybr143cp
Alias Sal4p , Sup1p , Sup47p
ORF Classification Verified
Description Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p (1, 2, 3, 4, 5, 6)
Name Description SUPpressor
Gene Product eRF1
Experimental Data
Molecules/cell 13100 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 437
Molecular Weight (Da) 49,005
Isoelectric Point (pI) 4.77

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sup45p (InterPro)
Physical Interactions There are 79 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052FB8 | P12385
MIPS: YBR143C
NCBI: 135054 | 308153442 | 398365061 | 4593 | 468605 | 536444 | NP_009701.3
GenBank/EMBL/DDBJ: DAA07259.1 | M28042 | X04082 | X73531 | Z36012
Amino Acid Sequence (or in FASTA format)
 
       1  MDNEVEKNIE IWKVKKLVQS LEKARGNGTS MISLVIPPKG QIPLYQKMLT
      51  DEYGTASNIK SRVNRLSVLS AITSTQQKLK LYNTLPKNGL VLYCGDIITE
     101  DGKEKKVTFD IEPYKPINTS LYLCDNKFHT EVLSELLQAD DKFGFIVMDG
     151  QGTLFGSVSG NTRTVLHKFT VDLPKKHGRG GQSALRFARL REEKRHNYVR
     201  KVAEVAVQNF ITNDKVNVKG LILAGSADFK TDLAKSELFD PRLACKVISI
     251  VDVSYGGENG FNQAIELSAE ALANVKYVQE KKLLEAYFDE ISQDTGKFCY
     301  GIDDTLKALD LGAVEKLIVF ENLETIRYTF KDAEDNEVIK FAEPEAKDKS
     351  FAIDKATGQE MDVVSEEPLI EWLAANYKNF GATLEFITDK SSEGAQFVTG
     401  FGGIGAMLRY KVNFEQLVDE SEDEYYDEDE GSDYDFI*

external links for Sup45p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Sup45p
1) Stansfield I, et al.  (1992) Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination. Mol Microbiol 6(23):3469-78
2) Himmelfarb HJ, et al.  (1985) Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product. Mol Cell Biol 5(4):816-22
3) Stansfield I, et al.  (1995) The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J 14(17):4365-73
4) Valouev IA, et al.  (2004) Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis. Mol Microbiol 53(2):687-96
5) Heurgue-Hamard V, et al.  (2006) The Zinc Finger Protein Ynr046w Is Plurifunctional and a Component of the eRF1 Methyltransferase in Yeast. J Biol Chem 281(47):36140-8
6) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41