BMT2/YBR141C Protein Information

Standard Name	Bmt2p 1
Systematic Name	Ybr141cp
ORF Classification	Verified
Description	Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene (1, 2, 3)
Name Description	Base MethylTransferase of 25S RNA 1

Experimental Data
Molecules/cell	623 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	337
Molecular Weight (Da)	38,550
Isoelectric Point (pI)	10.28

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bmt2p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD38 \| P38278 MIPS: YBR141C NCBI: 330443457 \| 342187336 \| 536440 \| 547577 \| 586549 \| NP_009699.2 GenBank/EMBL/DDBJ: DAA07257.2 \| X78937 \| Z36010

Amino Acid Sequence (or in FASTA format)
1 MHSRKSKSIT GKRKQVGSNV TRVIKPQKTR RIIRRFHHLI NKRQSICKFL 51 CLKENLDDSN EEKNDKIIRL SIKGNVRLGK YYEDGKSQSF NDAMESQLLR 101 LHSLIKNESK SKDTSDLAVM YTLLGYIMNQ INKLGGLETY QIASQNGQLK 151 ERGGDTSKLL EKWIRSSFEN CPGAVALEIG SLSSGNRISR CALFRNVVRI 201 DLEEHEGVIK QDFMERPLPR NENDKFDLIS CSLVLNFVKN HRDRGAMCHR 251 MVKFLKPQGY IFIVLPQACV THSRYCDKTL LQNLLGSIGL IMLNSHQSNK 301 LYYCLYQLQV VPPQPSSFSK RIKVNDGPGL NNFGITL*

Amino Acid Sequence (or in FASTA format)

       1  MHSRKSKSIT GKRKQVGSNV TRVIKPQKTR RIIRRFHHLI NKRQSICKFL
      51  CLKENLDDSN EEKNDKIIRL SIKGNVRLGK YYEDGKSQSF NDAMESQLLR
     101  LHSLIKNESK SKDTSDLAVM YTLLGYIMNQ INKLGGLETY QIASQNGQLK
     151  ERGGDTSKLL EKWIRSSFEN CPGAVALEIG SLSSGNRISR CALFRNVVRI
     201  DLEEHEGVIK QDFMERPLPR NENDKFDLIS CSLVLNFVKN HRDRGAMCHR
     251  MVKFLKPQGY IFIVLPQACV THSRYCDKTL LQNLLGSIGL IMLNSHQSNK
     301  LYYCLYQLQV VPPQPSSFSK RIKVNDGPGL NNFGITL*

external links for Bmt2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Bmt2p

1)	Sharma S, et al. (2013) Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in Saccharomyces cerevisiae. Nucleic Acids Res ()
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Petrossian TC and Clarke SG (2009) Multiple Motif Scanning to identify methyltransferases from the yeast proteome. Mol Cell Proteomics 8(7):1516-26
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41