IRA1/YBR140C Protein Information

Standard Name	Ira1p 1
Systematic Name	Ybr140cp
Alias	Glc1p , Ppd1p
ORF Classification	Verified
Description	GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication (1, 2, 3)
Name Description	Inhibitory Regulator of the RAS-cAMP pathway 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	3,092
Molecular Weight (Da)	350,976
Isoelectric Point (pI)	6.54

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ira1p (InterPro)
Physical Interactions	There are 13 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012D874 \| P18963 MIPS: YBR140C NCBI: 295616 \| 398365033 \| 496870 \| 536438 \| 547576 \| 585325 \| NP_009698.3 GenBank/EMBL/DDBJ: DAA07256.1 \| M24378 \| X75891 \| X78937 \| Z36009

Amino Acid Sequence (or in FASTA format)
1 MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN 51 SCRSIILNIA ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV 101 LLRLLSDVFE YYWDQNNDFK KIRNDNYKPG FSSHRPNFHT SRPKHTRINP 151 ALATMLLCKI SKLKFNTRTL KVLQNMSHHL SGSATISKSS ILPDSQEFLQ 201 KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA PLLISHTSTE 251 LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA 301 FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL 351 LFDDVYSTFS VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK 401 QSSVNASGNV SPSQFSTGND ASPTSPMASL SSPLNTNILG YPLSPITSTL 451 GQANTSTSTT AATTKTDADT PSTMNTNNNN NNNNSANLNN IPQRIFSLDD 501 ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG VNNSQSQNDQ 551 SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY 601 RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK 651 FLTYLIRNLN GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS 701 KRFYNLLGQN LEVGTNWNSA TANTFISHCV ERNPLTHRRL QLEFFASGLQ 751 LDSDLFLRHL QLEKELNHID LPKISLYTEG FRVFFHLVST KKLHEDIAEK 801 TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL DQFDAARTLS 851 NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR 901 TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT 951 TKRYASPNDS ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA 1001 TPTILKNIKS PKPNKTKKIA DDKQLKQPSY SRVILSDNDE ARKIMMNIFS 1051 IFKRMTNWFI RPDANTEFPK TFTDIIKPLF VSILDSNQRL QVTARAFIEI 1101 PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD LKLENAKREM 1151 LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS 1201 LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD 1251 NFTASGSVAL QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT 1301 QEELVDFRSL AGILASMSGI LSDMQELEKS KSAPDNEGDS LSFESRNPAY 1351 EVHKSLKLEL TKKMNFFISK QCQWLNNPNL LTRENSRDIL SIELHPLSFN 1401 LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI LKRDDDEKIM 1451 LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG 1501 ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY 1551 IDTSIESAKA LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL 1601 EKSADLNQFP VSLRHKISIL NENVIIALTN LSNANVNVSL KFTLPMGYSP 1651 NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK MLAIDDFLKY IIKNPILAFF 1701 GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK RAARSDDILR 1751 RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN 1801 SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY 1851 IAVGSFVFLR FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR 1901 ENIFKKDILV SKEEFLKTCS DKIFNFLSEL CKIPTNNFTV NVREDPTPIS 1951 FDYSFLHKFF YLNEFTIRKE IINESKLPGE FSFLKNTVML NDKILGVLGQ 2001 PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE EEDNAPFVHE 2051 AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT 2101 TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV 2151 ENPYLVTTIP RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD 2201 KEKKKFCPVS LKIGNKYFQV LHEIPQLYKV TVSNRTFSIK FNNVYKISNL 2251 ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP KYLEIVKMFY YAQLKMEEDF 2301 GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV GLFNEDDLVK 2351 NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST 2401 ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG 2451 PEAISRIIYS LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH 2501 ALDRDSENRD WMKAVSILTS FPTTEIACQV IEKLINMIKS FLPSLAVEAS 2551 AHSWSELTIL SKISVSIFFE SPLLSQMYLP EILFAVSLLI DVGPSEIRVS 2601 LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF ISGFSQEKGR 2651 VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA 2701 IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM 2751 FIIIAHVFTY SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM 2801 VNCLKRLYTV HLQLGFDGKS LAKKLMESRN FAATLLAKLE SYNGCIWNED 2851 NFPHIILGFI ANGLSIPVVK GAALDCLQAL FKNTYYERKS NPKSSDYLCY 2901 LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI NWLSSDSDKS 2951 NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF 3001 RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV 3051 VKPLDIFDQE HIEKLKGEGE HQVAIYERKR LATMILARMS CS*

Amino Acid Sequence (or in FASTA format)

       1  MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN
      51  SCRSIILNIA ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV
     101  LLRLLSDVFE YYWDQNNDFK KIRNDNYKPG FSSHRPNFHT SRPKHTRINP
     151  ALATMLLCKI SKLKFNTRTL KVLQNMSHHL SGSATISKSS ILPDSQEFLQ
     201  KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA PLLISHTSTE
     251  LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA
     301  FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL
     351  LFDDVYSTFS VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK
     401  QSSVNASGNV SPSQFSTGND ASPTSPMASL SSPLNTNILG YPLSPITSTL
     451  GQANTSTSTT AATTKTDADT PSTMNTNNNN NNNNSANLNN IPQRIFSLDD
     501  ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG VNNSQSQNDQ
     551  SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY
     601  RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK
     651  FLTYLIRNLN GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS
     701  KRFYNLLGQN LEVGTNWNSA TANTFISHCV ERNPLTHRRL QLEFFASGLQ
     751  LDSDLFLRHL QLEKELNHID LPKISLYTEG FRVFFHLVST KKLHEDIAEK
     801  TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL DQFDAARTLS
     851  NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR
     901  TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT
     951  TKRYASPNDS ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA
    1001  TPTILKNIKS PKPNKTKKIA DDKQLKQPSY SRVILSDNDE ARKIMMNIFS
    1051  IFKRMTNWFI RPDANTEFPK TFTDIIKPLF VSILDSNQRL QVTARAFIEI
    1101  PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD LKLENAKREM
    1151  LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS
    1201  LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD
    1251  NFTASGSVAL QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT
    1301  QEELVDFRSL AGILASMSGI LSDMQELEKS KSAPDNEGDS LSFESRNPAY
    1351  EVHKSLKLEL TKKMNFFISK QCQWLNNPNL LTRENSRDIL SIELHPLSFN
    1401  LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI LKRDDDEKIM
    1451  LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG
    1501  ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY
    1551  IDTSIESAKA LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL
    1601  EKSADLNQFP VSLRHKISIL NENVIIALTN LSNANVNVSL KFTLPMGYSP
    1651  NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK MLAIDDFLKY IIKNPILAFF
    1701  GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK RAARSDDILR
    1751  RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN
    1801  SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY
    1851  IAVGSFVFLR FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR
    1901  ENIFKKDILV SKEEFLKTCS DKIFNFLSEL CKIPTNNFTV NVREDPTPIS
    1951  FDYSFLHKFF YLNEFTIRKE IINESKLPGE FSFLKNTVML NDKILGVLGQ
    2001  PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE EEDNAPFVHE
    2051  AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT
    2101  TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV
    2151  ENPYLVTTIP RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD
    2201  KEKKKFCPVS LKIGNKYFQV LHEIPQLYKV TVSNRTFSIK FNNVYKISNL
    2251  ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP KYLEIVKMFY YAQLKMEEDF
    2301  GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV GLFNEDDLVK
    2351  NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST
    2401  ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG
    2451  PEAISRIIYS LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH
    2501  ALDRDSENRD WMKAVSILTS FPTTEIACQV IEKLINMIKS FLPSLAVEAS
    2551  AHSWSELTIL SKISVSIFFE SPLLSQMYLP EILFAVSLLI DVGPSEIRVS
    2601  LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF ISGFSQEKGR
    2651  VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA
    2701  IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM
    2751  FIIIAHVFTY SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM
    2801  VNCLKRLYTV HLQLGFDGKS LAKKLMESRN FAATLLAKLE SYNGCIWNED
    2851  NFPHIILGFI ANGLSIPVVK GAALDCLQAL FKNTYYERKS NPKSSDYLCY
    2901  LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI NWLSSDSDKS
    2951  NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF
    3001  RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV
    3051  VKPLDIFDQE HIEKLKGEGE HQVAIYERKR LATMILARMS CS*

external links for Ira1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ira1p

1)	Tanaka K, et al. (1989) IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. Mol Cell Biol 9(2):757-68
2)	Mitts MR, et al. (1991) Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1. Mol Cell Biol 11(9):4591-8
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61