HSL7/YBR133C Protein Information

Standard Name	Hsl7p
Systematic Name	Ybr133cp
ORF Classification	Verified
Description	Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description	Histone Synthetic Lethal 8

Experimental Data
Molecules/cell	1180 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	827
Molecular Weight (Da)	95,152
Isoelectric Point (pI)	6.32

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hsl7p (InterPro)
Physical Interactions	There are 34 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F8DB \| P38274 MIPS: YBR133C NCBI: 1519245 \| 496864 \| 536425 \| 586543 \| 6319610 \| NP_009691.1 \| NM_001178481.1 GenBank/EMBL/DDBJ: DAA07250.1 \| U65920 \| X75891 \| Z36002
External Classifications	EC: 2.1.1.125 [Histone-arginine N-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MHSNVFVGVK PGFNHKQHSK KSRFLENVSS HSPELPSNYD YVLLPITTPR 51 YKEIVGQVFK DFQRQSIQNW KPLQIPEPQL QDICIPPFNV KKLDNDDTPS 101 YIGLLSSWLE LESRDPNVRD LGLKVLLNEC KYARFVGINK LILAPPRDLS 151 NLQLYGQMIY RLLQNRIVFA APALTISISL PLYEDSDPLA TWELWNTVRK 201 QCEYHPSLTI SLALPRTRTP SYVLNRWLAE PVSCLLVSSS IFASNQYDYP 251 VLHKFNQNLI LKFQKVNGDS QILGNELCVI LHGMEKYANN VKGGESAYLE 301 YINYLLKKGD KVLNSNSNHQ FLLQEDSRIM PPLKPHSDNL LNSTYLTFEK 351 DLVKYDLYES AILEALQDLA PRASAKRPLV ILVAGAGRGP LVDRTFKIIS 401 MLFMDSKVSI IAIEKNPQAY LYLQKRNFDC WDNRVKLIKE DMTKWQINEP 451 SEKRIQIDLC ISELLGSFGC NELSPECLWS IEKYHSHNDT IFIPRSYSSY 501 IAPISSPLFY QKLSQTNRSL EAPWIVHRVP YCILSSRVNE VWRFEHPMAQ 551 KDTVQDEDDF TVEFSQSSLN EFKIKHRGEI HGFIGFFSAN LYNNIFLSTL 601 PNDSTVRLKF SEETLMNTRR EENLIKKCDH TPNMTSWSPI IFPLKQPISF 651 IDDSELSVLM SRIHSDTEQK VWYEWSLESF IYLMLSNYTS AVTAASMTIP 701 RSIVTDDTKT LAHNRHYSAT TNQKLDNQID LDQDIENEEE QGFLSNLETG 751 WQSVQDIHGL SETAKPDHLD SINKPMFDLK STKALEPSNE LPRHEDLEED 801 VPEVHVRVKT SVSTLHNVCG RAFSLPL*

Amino Acid Sequence (or in FASTA format)

       1  MHSNVFVGVK PGFNHKQHSK KSRFLENVSS HSPELPSNYD YVLLPITTPR
      51  YKEIVGQVFK DFQRQSIQNW KPLQIPEPQL QDICIPPFNV KKLDNDDTPS
     101  YIGLLSSWLE LESRDPNVRD LGLKVLLNEC KYARFVGINK LILAPPRDLS
     151  NLQLYGQMIY RLLQNRIVFA APALTISISL PLYEDSDPLA TWELWNTVRK
     201  QCEYHPSLTI SLALPRTRTP SYVLNRWLAE PVSCLLVSSS IFASNQYDYP
     251  VLHKFNQNLI LKFQKVNGDS QILGNELCVI LHGMEKYANN VKGGESAYLE
     301  YINYLLKKGD KVLNSNSNHQ FLLQEDSRIM PPLKPHSDNL LNSTYLTFEK
     351  DLVKYDLYES AILEALQDLA PRASAKRPLV ILVAGAGRGP LVDRTFKIIS
     401  MLFMDSKVSI IAIEKNPQAY LYLQKRNFDC WDNRVKLIKE DMTKWQINEP
     451  SEKRIQIDLC ISELLGSFGC NELSPECLWS IEKYHSHNDT IFIPRSYSSY
     501  IAPISSPLFY QKLSQTNRSL EAPWIVHRVP YCILSSRVNE VWRFEHPMAQ
     551  KDTVQDEDDF TVEFSQSSLN EFKIKHRGEI HGFIGFFSAN LYNNIFLSTL
     601  PNDSTVRLKF SEETLMNTRR EENLIKKCDH TPNMTSWSPI IFPLKQPISF
     651  IDDSELSVLM SRIHSDTEQK VWYEWSLESF IYLMLSNYTS AVTAASMTIP
     701  RSIVTDDTKT LAHNRHYSAT TNQKLDNQID LDQDIENEEE QGFLSNLETG
     751  WQSVQDIHGL SETAKPDHLD SINKPMFDLK STKALEPSNE LPRHEDLEED
     801  VPEVHVRVKT SVSTLHNVCG RAFSLPL*

external links for Hsl7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Hsl7p

1)	McMillan JN, et al. (1999) The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p. Mol Cell Biol 19(10):6929-39
2)	Shulewitz MJ, et al. (1999) Hsl7 localizes to a septin ring and serves as an adapter in a regulatory pathway that relieves tyrosine phosphorylation of Cdc28 protein kinase in Saccharomyces cerevisiae. Mol Cell Biol 19(10):7123-37
3)	Longtine MS, et al. (2000) Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol 20(11):4049-61
4)	Lee JH, et al. (2000) Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase. Biochem Biophys Res Commun 274(1):105-11
5)	Cid VJ, et al. (2001) Dynamic localization of the Swe1 regulator Hsl7 during the Saccharomyces cerevisiae cell cycle. Mol Biol Cell 12(6):1645-69
6)	Sakchaisri K, et al. (2004) Coupling morphogenesis to mitotic entry. Proc Natl Acad Sci U S A 101(12):4124-9
7)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8)	Ma XJ, et al. (1996) A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae. Genes Dev 10(11):1327-40
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41