TKL2/YBR117C Protein Information

Standard Name	Tkl2p
Systematic Name	Ybr117cp
ORF Classification	Verified
Description	Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication (1, 2)
Name Description	TransKetoLase

Experimental Data
Molecules/cell	149 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	681
Molecular Weight (Da)	75,029
Isoelectric Point (pI)	6.07

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tkl2p (InterPro)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E91 \| P33315 MIPS: YBR117C NCBI: 313262 \| 398364879 \| 464894 \| 476071 \| 536458 \| NP_009675.3 GenBank/EMBL/DDBJ: DAA07235.1 \| X73532 \| X78993 \| Z35985
External Classifications	EC: 2.2.1.1 [Transketolase]

Amino Acid Sequence (or in FASTA format)
1 MAQFSDIDKL AVSTLRLLSV DQVESAQSGH PGAPLGLAPV AHVIFKQLRC 51 NPNNEHWINR DRFVLSNGHS CALLYSMLHL LGYDYSIEDL RQFRQVNSRT 101 PGHPEFHSAG VEITSGPLGQ GISNAVGMAI AQANFAATYN EDGFPISDSY 151 TFAIVGDGCL QEGVSSETSS LAGHLQLGNL ITFYDSNSIS IDGKTSYSFD 201 EDVLKRYEAY GWEVMEVDKG DDDMESISSA LEKAKLSKDK PTIIKVTTTI 251 GFGSLQQGTA GVHGSALKAD DVKQLKKRWG FDPNKSFVVP QEVYDYYKKT 301 VVEPGQKLNE EWDRMFEEYK TKFPEKGKEL QRRLNGELPE GWEKHLPKFT 351 PDDDALATRK TSQQVLTNMV QVLPELIGGS ADLTPSNLTR WEGAVDFQPP 401 ITQLGNYAGR YIRYGVREHG MGAIMNGISA FGANYKPYGG TFLNFVSYAA 451 GAVRLAALSG NPVIWVATHD SIGLGEDGPT HQPIETLAHL RAIPNMHVWR 501 PADGNETSAA YYSAIKSGRT PSVVALSRQN LPQLEHSSFE KALKGGYVIH 551 DVENPDIILV STGSEVSISI DAAKKLYDTK KIKARVVSLP DFYTFDRQSE 601 EYRFSVLPDG VPIMSFEVLA TSSWGKYAHQ SFGLDEFGRS GKGPEIYKLF 651 DFTADGVASR AEKTINYYKG KQLLSPMGRA F*

Amino Acid Sequence (or in FASTA format)

       1  MAQFSDIDKL AVSTLRLLSV DQVESAQSGH PGAPLGLAPV AHVIFKQLRC
      51  NPNNEHWINR DRFVLSNGHS CALLYSMLHL LGYDYSIEDL RQFRQVNSRT
     101  PGHPEFHSAG VEITSGPLGQ GISNAVGMAI AQANFAATYN EDGFPISDSY
     151  TFAIVGDGCL QEGVSSETSS LAGHLQLGNL ITFYDSNSIS IDGKTSYSFD
     201  EDVLKRYEAY GWEVMEVDKG DDDMESISSA LEKAKLSKDK PTIIKVTTTI
     251  GFGSLQQGTA GVHGSALKAD DVKQLKKRWG FDPNKSFVVP QEVYDYYKKT
     301  VVEPGQKLNE EWDRMFEEYK TKFPEKGKEL QRRLNGELPE GWEKHLPKFT
     351  PDDDALATRK TSQQVLTNMV QVLPELIGGS ADLTPSNLTR WEGAVDFQPP
     401  ITQLGNYAGR YIRYGVREHG MGAIMNGISA FGANYKPYGG TFLNFVSYAA
     451  GAVRLAALSG NPVIWVATHD SIGLGEDGPT HQPIETLAHL RAIPNMHVWR
     501  PADGNETSAA YYSAIKSGRT PSVVALSRQN LPQLEHSSFE KALKGGYVIH
     551  DVENPDIILV STGSEVSISI DAAKKLYDTK KIKARVVSLP DFYTFDRQSE
     601  EYRFSVLPDG VPIMSFEVLA TSSWGKYAHQ SFGLDEFGRS GKGPEIYKLF
     651  DFTADGVASR AEKTINYYKG KQLLSPMGRA F*

external links for Tkl2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tkl2p

1)	Schaaff-Gerstenschlager I, et al. (1993) TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene. Eur J Biochem 217(1):487-92
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41