ALG1/YBR110W Protein Information

Standard Name	Alg1p
Systematic Name	Ybr110wp
ORF Classification	Verified
Description	Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog (1, 2)
Name Description	Asparagine-Linked Glycosylation 3

Experimental Data
Molecules/cell	1890 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	449
Molecular Weight (Da)	51,929
Isoelectric Point (pI)	9.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Alg1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 37 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125835 \| P16661 MIPS: YBR110W NCBI: 113627 \| 171050 \| 398364841 \| 476066 \| 536377 \| NP_009668.3 GenBank/EMBL/DDBJ: DAA07229.1 \| J05416 \| X78993 \| Z35979
External Classifications	EC: 2.4.1.142 [Chitobiosyldiphosphodolichol beta-mannosyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG 51 HSPRICYHAI SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK 101 GGGTSVIFMV KKVLFQVLSI FKLLWELRGS DYILVQNPPS IPILPIAVLY 151 KLTGCKLIID WHNLAYSILQ LKFKGNFYHP LVLISYMVEM IFSKFADYNL 201 TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS RQKALTTKAF 251 IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS 301 LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD 351 YGVSLHTSSS GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV 401 DRRELHESLI FAMKDADLYQ KLKKNVTQEA ENRWQSNWER TMRDLKLIH*

Amino Acid Sequence (or in FASTA format)

       1  MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG
      51  HSPRICYHAI SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK
     101  GGGTSVIFMV KKVLFQVLSI FKLLWELRGS DYILVQNPPS IPILPIAVLY
     151  KLTGCKLIID WHNLAYSILQ LKFKGNFYHP LVLISYMVEM IFSKFADYNL
     201  TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS RQKALTTKAF
     251  IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS
     301  LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD
     351  YGVSLHTSSS GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV
     401  DRRELHESLI FAMKDADLYQ KLKKNVTQEA ENRWQSNWER TMRDLKLIH*

external links for Alg1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Alg1p

1)	Albright CF and Robbins RW (1990) The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation. J Biol Chem 265(12):7042-9
2)	Takahashi T, et al. (2000) Cloning of the human cDNA which can complement the defect of the yeast mannosyltransferase I-deficient mutant alg 1. Glycobiology 10(3):321-7
3)	Huffaker TC and Robbins PW (1982) Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J Biol Chem 257(6):3203-10
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41