MMS4/YBR098W Protein Information Help

Standard Name Mms4p 1
Systematic Name Ybr098wp
Alias Slx2p , Ybr100wp
ORF Classification Verified
Description Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p (1, 2, 3, 4, 5, 6)
Name Description Methyl MethaneSulfonate sensitivity 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 691
Molecular Weight (Da) 78,763
Isoelectric Point (pI) 7.53

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mms4p (InterPro)
Physical Interactions There are 19 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006AF56 | P38257
MIPS: YBR098W
NCBI: 27808702 | 46397829 | 476056 | 51013313 | 536382 | 536464 | 6318279 | NP_009656.2
GenBank/EMBL/DDBJ: DAA07219.1 | AY692931 | U14000 | X78993 | Z35967 | Z35968
Amino Acid Sequence (or in FASTA format)
 
       1  MSQIVDFVED KDSRNDASIQ IIDGPSNVEI IALSESMDQD ECKRAHVSSA
      51  EMIPSSPQRK SVSNDVENVD LNKSIELSAP FFQDISISKL DDFSTTVNSI
     101  IDSSLRNENN AKGNAKKLLD DLISDEWSAD LESSGKKHNK SQYNLRDIAE
     151  KWGVQSLKNP EPIAVDCEYK TQGIGKTNSD ISDSPKSQIG AADILFDFPL
     201  SPVKHENPTE EKHNSIANEN SSPDNSLKPA GKQNHGEDGT SMAKRVYNKG
     251  EDEQEHLPKG KKRTIALSRT LINSTKLPDT VELNLSKFLD SSDSITTDVL
     301  STPAKGSNIV RTGSQPIFSN ANCFQEAKRS KTLTAEDPKC TKNTAREVSQ
     351  LENYIAYGQY YTREDSKNKI RHLLKENKNA FKRVNQIYRD NIKARSQMII
     401  EFSPSLLQLF KKGDSDLQQQ LAPAVVQSSY NDSMPLLRFL RKCDSIYDFS
     451  NDFYYPCDPK IVEENVLILY YDAQEFFEQY TSQKKELYRK IRFFSKNGKH
     501  VILILSDINK LKRAIFQLEN EKYKARVEQR LSGTEEALRP RSKKSSQVGK
     551  LGIKKFDLEQ RLRFIDREWH VKIHTVNSHM EFINSLPNLV SLIGKQRMDP
     601  AIRYMKYAHL NVKSAQDSTE TLKKTFHQIG RMPEMKANNV VSLYPSFQSL
     651  LEDIEKGRLQ SDNEGKYLMT EAVEKRLYKL FTCTDPNDTI E*

external links for Mms4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome

YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mms4p
1) Xiao W, et al.  (1998) Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. Mol Gen Genet 257(6):614-23
2) Kaliraman V, et al.  (2001) Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev 15(20):2730-40
3) Bastin-Shanower SA, et al.  (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23(10):3487-96
4) Oh SD, et al.  (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
5) Jessop L and Lichten M  (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
6) Gallo-Fernandez M, et al.  (2012) Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res 40(17):8325-35