MIS1/YBR084W Protein Information Help

Standard Name Mis1p 1
Systematic Name Ybr084wp
ORF Classification Verified
Description Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase (1)
Name Description MItochondrial C1-tetrahydrofolate Synthase 1
Experimental Data
Molecules/cell 11400 2
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 975
Molecular Weight (Da) 106,216
Isoelectric Point (pI) 9.16

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mis1p (InterPro)
Physical Interactions There are 61 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E38 | P09440
MIPS: YBR084W
NCBI: 115209 | 171953 | 536348 | 6319558 | NP_009640.1
GenBank/EMBL/DDBJ: DAA07203.1 | J03724 | Z35953
External Classifications EC: 1.5.1.5 [Methylenetetrahydrofolate dehydrogenase (NADP(+))]
EC: 3.5.4.9 [Methenyltetrahydrofolate cyclohydrolase]
EC: 6.3.4.3 [Formate--tetrahydrofolate ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MLSRLSLLSN SRAFQQARWR IYRLKVSPTV HASQYHILSG RKLAQSIREK
      51  ANDEIQAIKL KHPNFKPTLK IIQVGARPDS STYVRMKLKA SKDSNVDCII
     101  EKLPAEITEV ELLKKISDIN DDDSIHGLLI QLPLPRHLDE TTITNAVDFK
     151  KDVDGFHRYN AGELAKKGGK PYFIPCTPYG CMKLLEEAHV KLDGKNAVVL
     201  GRSSIVGNPI ASLLKNANAT VTVCHSHTRN IAEVVSQADI VIAACGIPQY
     251  VKSDWIKEGA VVIDVGINYV PDISKKSGQK LVGDVDFDSV KEKTSYITPV
     301  PGGVGPMTVA MLVSNVLLAA KRQFVESEKL PVIKPLPLHL ESPVPSDIDI
     351  SRAQSPKHIK QVAEELGIHS HELELYGHYK AKISPNIFKR LESRENGKYV
     401  LVAGITPTPL GEGKSTTTMG LVQALSAHLG KPSIANVRQP SLGPTLGVKG
     451  GAAGGGYAQV IPMDEFNLHL TGDIHAISAA NNLLAAAIDT RMFHEATQKN
     501  DSTFYKRLVP RKKGIRKFTP SMQRRLKRLD IEKEDPDALT PEEVKRFARL
     551  NINPDTITIR RVVDINDRML RQITIGEAAT EKGFTRTTGF DITVASELMA
     601  ILALSKSLHE MKERIGRMVI GADYDNKPVT VEDIGCTGAL TALLRDAIKP
     651  NLMQTLEGTP VMVHAGPFAN ISIGASSVIA DLMALKLVGS EKNPLNDKNI
     701  HEPGYVVTEA GFDFAMGGER FFDIKCRSSG LVPDAVVLVA TVRALKSHGG
     751  APNVKPGQSL PKEYTEENID FVAKGVSNLV KQIENIKTFG IPVVVAINRF
     801  ETDSQAEIEV IKKAALNAGA SHAVTSNHWM EGGKGAVELA HAVVDATKEP
     851  KNFNFLYDVN SSIEDKLTSI VQKMYGGAKI EVSPEAQKKI DTYKKQGFGN
     901  LPICIAKTQY SLSHDPSLKG VPRGFTFPIR DVRASIGAGY LYALAAEIQT
     951  IPGLSTYAGY MAVEVDDDGE IEGLF*

external links for Mis1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Mis1p
1) Shannon KW and Rabinowitz JC  (1988) Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase. J Biol Chem 263(16):7717-25
2) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41