ECM33/YBR078W Protein Information

Standard Name	Ecm33p 1
Systematic Name	Ybr078wp
ORF Classification	Verified
Description	GPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	ExtraCellular Mutant 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	429
Molecular Weight (Da)	43,768
Isoelectric Point (pI)	4.48

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ecm33p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001722CFC \| P38248 MIPS: YBR078W NCBI: 1351738 \| 1872127 \| 1872129 \| 187608884 \| 330443439 \| 974204 \| NP_009634.2 GenBank/EMBL/DDBJ: DAA07197.2 \| X76294 \| Z35947 \| Z35948

Amino Acid Sequence (or in FASTA format)
1 MQFKNALTAT AILSASALAA NSTTSIPSSC SIGTSATATA QADLDKISGC 51 STIVGNLTIT GDLGSAALAS IQEIDGSLTI FNSSSLSSFS ADSIKKITGD 101 LNMQELIILT SASFGSLQEV DSINMVTLPA ISTFSTDLQN ANNIIVSDTT 151 LESVEGFSTL KKVNVFNINN NRYLNSFQSS LESVSDSLQF SSNGDNTTLA 201 FDNLVWANNI TLRDVNSISF GSLQTVNASL GFINNTLPSL NLTQLSKVGQ 251 SLSIVSNDEL SKAAFSNLTT VGGGFIIANN TQLKVIDGFN KVQTVGGAIE 301 VTGNFSTLDL SSLKSVRGGA NFDSSSSNFS CNALKKLQSN GAIQGDSFVC 351 KNGATSTSVK LSSTSTESSK SSATSSASSS GDASNAQANV SASASSSSSS 401 SKKSKGAAPE LVPATSFMGV VAAVGVALL*

Amino Acid Sequence (or in FASTA format)

       1  MQFKNALTAT AILSASALAA NSTTSIPSSC SIGTSATATA QADLDKISGC
      51  STIVGNLTIT GDLGSAALAS IQEIDGSLTI FNSSSLSSFS ADSIKKITGD
     101  LNMQELIILT SASFGSLQEV DSINMVTLPA ISTFSTDLQN ANNIIVSDTT
     151  LESVEGFSTL KKVNVFNINN NRYLNSFQSS LESVSDSLQF SSNGDNTTLA
     201  FDNLVWANNI TLRDVNSISF GSLQTVNASL GFINNTLPSL NLTQLSKVGQ
     251  SLSIVSNDEL SKAAFSNLTT VGGGFIIANN TQLKVIDGFN KVQTVGGAIE
     301  VTGNFSTLDL SSLKSVRGGA NFDSSSSNFS CNALKKLQSN GAIQGDSFVC
     351  KNGATSTSVK LSSTSTESSK SSATSSASSS GDASNAQANV SASASSSSSS
     401  SKKSKGAAPE LVPATSFMGV VAAVGVALL*

external links for Ecm33p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ecm33p

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Bidlingmaier S and Snyder M (2002) Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics 1(6):345-56
3)	Toh-e A and Oguchi T (2002) Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Genes Genet Syst 77(5):309-22
4)	Terashima H, et al. (2003) The localization change of Ybr078w/Ecm33, a yeast GPI-associated protein, from the plasma membrane to the cell wall, affecting the cellular function. FEMS Microbiol Lett 218(1):175-80
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906