RDH54/YBR073W Protein Information

Standard Name	Rdh54p 1
Systematic Name	Ybr073wp
Alias	Tid1p 2
ORF Classification	Verified
Description	DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p (1, 2, 3, 4, 5)
Name Description	RaD54 Homolog 1

Experimental Data
Molecules/cell	1270 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	958
Molecular Weight (Da)	107,946
Isoelectric Point (pI)	9.68

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rdh54p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD33 \| P38086 MIPS: YBR073W NCBI: 341942271 \| 398364627 \| 433837 \| 536326 \| 78099794 \| NP_009629.6 GenBank/EMBL/DDBJ: DAA07193.2 \| X76294 \| Z35942
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase] EC: 5.99.-.- [Other Isomerases] EC: 5.99.1.-

Amino Acid Sequence (or in FASTA format)
1 MAVISVKPRR REKILQEVKN SSVYQTVFDS GTTQMQIPKY ENKPFKPPRR 51 VGSNKYTQLK PTATAVTTAP ISKAKVTVNL KRSISAGPTL NLAKKPNNLS 101 SNENTRYFTI MYRKPTTKKH KTWSGDGYAT LKASSDKLCF YNEAGKFLGS 151 SMLPSDSDSL FETLFKAGSN EVQLDYELKE NAEIRSAKEA LSQNMGNPSP 201 PTTSTTETVP STKNDGGKYQ MPLSQLFSLN TVKRFKSVTK QTNEHMTTVP 251 KTSQNSKAKK YYPVFDVNKI DNPIVMNKNA AAEVDVIVDP LLGKFLRPHQ 301 REGVKFMYDC LMGLARPTIE NPDIDCTTKS LVLENDSDIS GCLLADDMGL 351 GKTLMSITLI WTLIRQTPFA SKVSCSQSGI PLTGLCKKIL VVCPVTLIGN 401 WKREFGKWLN LSRIGVLTLS SRNSPDMDKM AVRNFLKVQR TYQVLIIGYE 451 KLLSVSEELE KNKHLIDMLV CDEGHRLKNG ASKILNTLKS LDIRRKLLLT 501 GTPIQNDLNE FFTIIDFINP GILGSFASFK RRFIIPITRA RDTANRYNEE 551 LLEKGEERSK EMIEITKRFI LRRTNAILEK YLPPKTDIIL FCKPYSQQIL 601 AFKDILQGAR LDFGQLTFSS SLGLITLLKK VCNSPGLVGS DPYYKSHIKD 651 TQSQDSYSRS LNSGKLKVLM TLLEGIRKGT KEKVVVVSNY TQTLDIIENL 701 MNMAGMSHCR LDGSIPAKQR DSIVTSFNRN PAIFGFLLSA KSGGVGLNLV 751 GASRLILFDN DWNPSVDLQA MSRIHRDGQK KPCFIYRLVT TGCIDEKILQ 801 RQLMKNSLSQ KFLGDSEMRN KESSNDDLFN KEDLKDLFSV HTDTKSNTHD 851 LICSCDGLGE EIEYPETNQQ QNTVELRKRS TTTWTSALDL QKKMNEAATN 901 DDAKKSQYIR QCLVHYKHID PARQDELFDE VITDSFTELK DSITFAFVKP 951 GEICLREQ*

Amino Acid Sequence (or in FASTA format)

       1  MAVISVKPRR REKILQEVKN SSVYQTVFDS GTTQMQIPKY ENKPFKPPRR
      51  VGSNKYTQLK PTATAVTTAP ISKAKVTVNL KRSISAGPTL NLAKKPNNLS
     101  SNENTRYFTI MYRKPTTKKH KTWSGDGYAT LKASSDKLCF YNEAGKFLGS
     151  SMLPSDSDSL FETLFKAGSN EVQLDYELKE NAEIRSAKEA LSQNMGNPSP
     201  PTTSTTETVP STKNDGGKYQ MPLSQLFSLN TVKRFKSVTK QTNEHMTTVP
     251  KTSQNSKAKK YYPVFDVNKI DNPIVMNKNA AAEVDVIVDP LLGKFLRPHQ
     301  REGVKFMYDC LMGLARPTIE NPDIDCTTKS LVLENDSDIS GCLLADDMGL
     351  GKTLMSITLI WTLIRQTPFA SKVSCSQSGI PLTGLCKKIL VVCPVTLIGN
     401  WKREFGKWLN LSRIGVLTLS SRNSPDMDKM AVRNFLKVQR TYQVLIIGYE
     451  KLLSVSEELE KNKHLIDMLV CDEGHRLKNG ASKILNTLKS LDIRRKLLLT
     501  GTPIQNDLNE FFTIIDFINP GILGSFASFK RRFIIPITRA RDTANRYNEE
     551  LLEKGEERSK EMIEITKRFI LRRTNAILEK YLPPKTDIIL FCKPYSQQIL
     601  AFKDILQGAR LDFGQLTFSS SLGLITLLKK VCNSPGLVGS DPYYKSHIKD
     651  TQSQDSYSRS LNSGKLKVLM TLLEGIRKGT KEKVVVVSNY TQTLDIIENL
     701  MNMAGMSHCR LDGSIPAKQR DSIVTSFNRN PAIFGFLLSA KSGGVGLNLV
     751  GASRLILFDN DWNPSVDLQA MSRIHRDGQK KPCFIYRLVT TGCIDEKILQ
     801  RQLMKNSLSQ KFLGDSEMRN KESSNDDLFN KEDLKDLFSV HTDTKSNTHD
     851  LICSCDGLGE EIEYPETNQQ QNTVELRKRS TTTWTSALDL QKKMNEAATN
     901  DDAKKSQYIR QCLVHYKHID PARQDELFDE VITDSFTELK DSITFAFVKP
     951  GEICLREQ*

external links for Rdh54p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rdh54p

1)	Klein HL (1997) RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Genetics 147(4):1533-43
2)	Dresser ME, et al. (1997) DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics 147(2):533-44
3)	Petukhova G, et al. (2000) Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes Dev 14(17):2206-15
4)	Shinohara M, et al. (2003) Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Genetics 163(4):1273-86
5)	Nimonkar AV, et al. (2012) Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem 287(34):28727-37
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41