CHS2/YBR038W Protein Information Help

Standard Name Chs2p 1
Systematic Name Ybr038wp
ORF Classification Verified
Description Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis (2, 3, 4, 5, 6)
Name Description CHitin Synthase 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 963
Molecular Weight (Da) 109,881
Isoelectric Point (pI) 8.98

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Chs2p (InterPro)
Transmembrane Domains There are 7 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 16 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127907 | P14180
MIPS: YBR038W
NCBI: 116382 | 171220 | 536258 | 6319512 | NP_009594.1
GenBank/EMBL/DDBJ: DAA07158.1 | M23865 | Z35907
External Classifications EC: 2.4.1.16 [Chitin synthase]
Amino Acid Sequence (or in FASTA format)
 
       1  MTRNPFMVEP SNGSPNRRGA SNLSKFYANA NSNSRWANPS EESLEDSYDQ
      51  SNVFQGLPAS PSRAALRYSP DRRHRTQFYR DSAHNSPVAP NRYAANLQES
     101  PKRAGEAVIH LSEGSNLYPR DNADLPVDPY HLSPQQQPSN NLFGSGRLYS
     151  QSSKYTMSTT STTAPSLAEA DDEKEKYLTS TTSYDDQSTI FSADTFNETK
     201  FELNHPTRQQ YVRRANSESK RRMVSDLPPP SKKKALLKLD NPIPKGLLDT
     251  LPRRNSPEFT EMRYTACTVE PDDFLREGYT LRFAEMNREC QIAICITMYN
     301  EDKYSLARTI HSIMKNVAHL CKREKSHVWG PNGWKKVSVI LISDGRAKVN
     351  QGSLDYLAAL GVYQEDMAKA SVNGDPVKAH IFELTTQVSI NADLDYVSKD
     401  IVPVQLVFCL KEENKKKINS HRWLFNAFCP VLQPTVVTLV DVGTRLNNTA
     451  IYRLWKVFDM DSNVAGAAGQ IKTMKGKWGL KLFNPLVASQ NFEYKISNIL
     501  DKPLESVFGY ISVLPGALSA YRYRALKNHE DGTGPLRSYF LGETQEGRDH
     551  DVFTANMYLA EDRILCWELV AKRDAKWVLK YVKEATGETD VPEDVSEFIS
     601  QRRRWLNGAM FAAIYAQLHF YQIWKTKHSV VRKFFLHVEF LYQFIQMLFS
     651  WFSIANFVLT FYYLAGSMNL VIKHGEALFI FFKYLIFCDL ASLFIISMGN
     701  RPQGAKHLFI TSMVILSICA TYSLICGFVF AFKSLASGTE SHKIFVDIVI
     751  SLLSTYGLYF FSSLMYLDPW HMFTSSIQYF LTLPAFTCTL QIFAFCNTHD
     801  VSWGTKGSTQ ESKQLSKAIV VQGPDGKQIV ETDWPQEVDK KFLEIKSRLK
     851  EPEFEESSGN EKQSKNDYYR DIRTRIVMIW MLSNLILIMS IIQVFTPQDT
     901  DNGYLIFILW SVAALAAFRV VGSMAFLFMK YLRIIVSYRN KVEGSGSWEV
     951  SKLDLPNVFH KKG*

external links for Chs2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Chs2p
1) Sburlati A and Cabib E  (1986) Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae. J Biol Chem 261(32):15147-52
2) Shaw JA, et al.  (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114(1):111-23
3) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
4) Schmidt M, et al.  (2002) In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other. J Cell Sci 115(Pt 2):293-302
5) Teh EM, et al.  (2009) Retention of Chs2p in the ER requires N-terminal CDK1-phosphorylation sites. Cell Cycle 8(18):2964-74
6) Oh Y, et al.  (2012) Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell 23(13):2445-56