YPK3/YBR028C Protein Information

Standard Name	Ypk3p 1
Systematic Name	Ybr028cp
ORF Classification	Verified
Description	An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner (1, 2, 3)

Experimental Data
Molecules/cell	1470 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	525
Molecular Weight (Da)	59,591
Isoelectric Point (pI)	7.87

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ypk3p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012DC59 \| P38070 MIPS: YBR028C NCBI: 498756 \| 536371 \| 585347 \| 6319502 \| NP_009584.1 GenBank/EMBL/DDBJ: DAA07149.1 \| X76078 \| Z35897
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MIFSLDEELH RVSLDDKKND IKVDYSSAIY NDINHEQGSS ITYEESINHL 51 SVHSNAIPLN GMSPAHRMRR RSSAYSKFPI LTPPNTRRFS ITGSDAMRTN 101 TNRLSITPQD IISSNIGENE LSRNLHDFKP VRVLGQGAYG KVLLVKDVNT 151 SKLYAMKQLR KAEILISQTA TDSKREDEDK NDGNNNDNDD GLSKRLERTF 201 AERSILSEIE HPNIVKLFYS FHDNSKLYLL LQYIPGGELF YHLKEHGTLD 251 ETTVSFYAAE ISCALRFLHT KGVVYRDLKP ENCLLNQRGH LVLTDFGLSK 301 KSANDSAVDE EDPENVNALY SIIGTPEYCA PEILLGKAYS QNCDWYSLGC 351 LLYDMLVGKP PYTGSNHKVI INKIQQNKQG PKIPFYLSEG MKDILNALLK 401 KETAKRWNVD KYWAKTGANN KPTKSKKKKS GAARTSLFTE HFIFRKIDWK 451 LLESGQLQKT TLGPIVPVIT DLELAENFDT EFTSMSYEET YTDSKPININ 501 SVSKSPDMFK GFSYKASGSY LEKYF*

Amino Acid Sequence (or in FASTA format)

       1  MIFSLDEELH RVSLDDKKND IKVDYSSAIY NDINHEQGSS ITYEESINHL
      51  SVHSNAIPLN GMSPAHRMRR RSSAYSKFPI LTPPNTRRFS ITGSDAMRTN
     101  TNRLSITPQD IISSNIGENE LSRNLHDFKP VRVLGQGAYG KVLLVKDVNT
     151  SKLYAMKQLR KAEILISQTA TDSKREDEDK NDGNNNDNDD GLSKRLERTF
     201  AERSILSEIE HPNIVKLFYS FHDNSKLYLL LQYIPGGELF YHLKEHGTLD
     251  ETTVSFYAAE ISCALRFLHT KGVVYRDLKP ENCLLNQRGH LVLTDFGLSK
     301  KSANDSAVDE EDPENVNALY SIIGTPEYCA PEILLGKAYS QNCDWYSLGC
     351  LLYDMLVGKP PYTGSNHKVI INKIQQNKQG PKIPFYLSEG MKDILNALLK
     401  KETAKRWNVD KYWAKTGANN KPTKSKKKKS GAARTSLFTE HFIFRKIDWK
     451  LLESGQLQKT TLGPIVPVIT DLELAENFDT EFTSMSYEET YTDSKPININ
     501  SVSKSPDMFK GFSYKASGSY LEKYF*

external links for Ypk3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ypk3p

1)	Soulard A, et al. (2010) The Rapamycin-sensitive Phosphoproteome Reveals That TOR Controls Protein Kinase A Toward Some But Not All Substrates. Mol Biol Cell 21(19):3475-86
2)	Zhu H, et al. (2000) Analysis of yeast protein kinases using protein chips. Nat Genet 26(3):283-9
3)	Budovskaya YV, et al. (2005) An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase. Proc Natl Acad Sci U S A 102(39):13933-8
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41