OLA1/YBR025C Protein Information

Standard Name	Ola1p 1
Systematic Name	Ybr025cp
ORF Classification	Verified
Description	P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress (1, 2, 3, 4)
Name Description	Obg-Like ATPase 1

Experimental Data
Molecules/cell	36800 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	394
Molecular Weight (Da)	44,174
Isoelectric Point (pI)	7.42

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ola1p (InterPro)
Physical Interactions	There are 47 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053086 \| P38219 MIPS: YBR025C NCBI: 498754 \| 536234 \| 586477 \| 6319499 \| NP_009581.1 GenBank/EMBL/DDBJ: DAA07147.1 \| X76078 \| Z35894

Amino Acid Sequence (or in FASTA format)
1 MPPKKQVEEK KVLLGRPGNN LKAGIVGLAN VGKSTFFQAI TRCPLGNPAN 51 YPFATIDPEE ARVIVPSPRF DKLCEIYKKT ASEVPAHLTV YDIAGLTKGA 101 SAGEGLGNAF LSHIRSVDSI YQVVRCFDDA EIIHVEGDVD PVRDLEIINQ 151 ELRLKDIEFA QKALEGAEKI AKRGGQSLEV KQKKEEMDLI TKIIKLLESG 201 QRVANHSWTS KEVEIINSMF LLTAKPCIYL INLSERDYIR KKNKHLLRIK 251 EWVDKYSPGD LIIPFSVSLE ERLSHMSPED AEEELKKLQT ISALPKIITT 301 MRQKLDLISF FTCGPDEVRE WTIRRGTKAP QAAGVIHNDL MNTFILAQVM 351 KCEDVFEYKD DSAIKAAGKL MQKGKDYVVE DGDIIYFRAG AGKN*

Amino Acid Sequence (or in FASTA format)

       1  MPPKKQVEEK KVLLGRPGNN LKAGIVGLAN VGKSTFFQAI TRCPLGNPAN
      51  YPFATIDPEE ARVIVPSPRF DKLCEIYKKT ASEVPAHLTV YDIAGLTKGA
     101  SAGEGLGNAF LSHIRSVDSI YQVVRCFDDA EIIHVEGDVD PVRDLEIINQ
     151  ELRLKDIEFA QKALEGAEKI AKRGGQSLEV KQKKEEMDLI TKIIKLLESG
     201  QRVANHSWTS KEVEIINSMF LLTAKPCIYL INLSERDYIR KKNKHLLRIK
     251  EWVDKYSPGD LIIPFSVSLE ERLSHMSPED AEEELKKLQT ISALPKIITT
     301  MRQKLDLISF FTCGPDEVRE WTIRRGTKAP QAAGVIHNDL MNTFILAQVM
     351  KCEDVFEYKD DSAIKAAGKL MQKGKDYVVE DGDIIYFRAG AGKN*

external links for Ola1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ola1p

1)	Koller-Eichhorn R, et al. (2007) Human OLA1 Defines an ATPase Subfamily in the Obg Family of GTP-binding Proteins. J Biol Chem 282(27):19928-37
2)	Godon C, et al. (1998) The H2O2 stimulon in Saccharomyces cerevisiae. J Biol Chem 273(35):22480-9
3)	Guerrero C, et al. (2006) An integrated mass spectrometry-based proteomic approach: quantitative analysis of tandem affinity-purified in vivo cross-linked protein complexes (QTAX) to decipher the 26 S proteasome-interacting network. Mol Cell Proteomics 5(2):366-78
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41