FLR1/YBR008C Protein Information Help

Standard Name Flr1p
Systematic Name Ybr008cp
ORF Classification Verified
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description FLuconazole Resistance 3
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 548
Molecular Weight (Da) 61,628
Isoelectric Point (pI) 6.31

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Flr1p (InterPro)
Transmembrane Domains There are 12 total predicted transmembrane domains (TMHMM)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012AA32 | P38124
MIPS: YBR008C
NCBI: 536200 | 586469 | 6319480 | NP_009562.1
GenBank/EMBL/DDBJ: DAA07129.1 | Z35877
External Classifications TC: 2.A.1.2 [The Drug:H+ Antiporter-1 (12 Spanner) (DHA1) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MVYTSTYRHT IVVDLLEYLG IVSNLETLQS AREDETRKPE NTDKKECKPD
      51  YDIECGPNRS CSESSTDSDS SGSQIEKNDP FRVDWNGPSD PENPQNWPLL
     101  KKSLVVFQIM LLTCVTYMGS SIYTPGQEYI QEEFHVGHVV ATLNLSLYVL
     151  GYGLGPIIFS PLSETARYGR LNLYMVTLFF FMIFQVGCAT VHNIGGLIVM
     201  RFISGILCSP SLATGGGTVA DIISPEMVPL VLGMWSAGAV AAPVLAPLLG
     251  AAMVDAKNWR FIFWLLMWLS AATFILLAFF FPETQHHNIL YRRALKLRKE
     301  TGDDRYYTEQ DKLDREVDAR TFLINTLYRP LKMIIKEPAI LAFDLYIAVA
     351  YGCFYLFFEA FPIVFVGIYH FSLVEVGLAY MGFCVGCVLA YGLFGILNMR
     401  IIVPRFRNGT FTPEAFLIVA MCVCWCLPLS LFLFGWTARV HWILPVISEV
     451  FFVLAVFNIF QATFAYLATC YPKYVASVFA GNGFCRASFA CAFPLFGRAM
     501  YDNLATKNYP VAWGSSLVGF LTLGLAIIPF ILYKYGPSLR TRSSYTEE*

external links for Flr1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome TCDB
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Flr1p
1) Broco N, et al.  (1999) FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiae and its benomyl-induced expression is dependent on pdr3 transcriptional regulator. Yeast 15(15):1595-608
2) Jungwirth H, et al.  (2000) Diazaborine resistance in yeast involves the efflux pumps Ycf1p and Flr1p and is enhanced by a gain-of-function allele of gene YAP1. Eur J Biochem 267(15):4809-16
3) Alarco AM, et al.  (1997) AP1-mediated multidrug resistance in Saccharomyces cerevisiae requires FLR1 encoding a transporter of the major facilitator superfamily. J Biol Chem 272(31):19304-13
4) Iwahashi Y, et al.  (2006) Mechanisms of patulin toxicity under conditions that inhibit yeast growth. J Agric Food Chem 54(5):1936-42
5) Dias PJ, et al.  (2010) Evolution of the 12-Spanner Drug:H+ Antiporter DHA1 Family in Hemiascomycetous Yeasts. OMICS 14(6):701-10
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76