ALG3/YBL082C Protein Information

Standard Name	Alg3p 1
Systematic Name	Ybl082cp
Alias	Rhk1p 2
ORF Classification	Verified
Description	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins (2, 3, 4)
Name Description	Asparagine Linked Glycosylation 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	458
Molecular Weight (Da)	52,860
Isoelectric Point (pI)	9.29

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Alg3p (InterPro)
Transmembrane Domains	There are 9 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125839 \| P38179 MIPS: YBL082C NCBI: 496697 \| 536134 \| 586444 \| 6319389 \| 984725 \| NP_009471.1 GenBank/EMBL/DDBJ: DAA07041.1 \| M89908 \| X79489 \| Z35844

Amino Acid Sequence (or in FASTA format)
1 MEGEQSPQGE KSLQRKQFVR PPLDLWQDLK DGVRYVIFDC RANLIVMPLL 51 ILFESMLCKI IIKKVAYTEI DYKAYMEQIE MIQLDGMLDY SQVSGGTGPL 101 VYPAGHVLIY KMMYWLTEGM DHVERGQVFF RYLYLLTLAL QMACYYLLHL 151 PPWCVVLACL SKRLHSIYVL RLFNDCFTTL FMVVTVLGAI VASRCHQRPK 201 LKKSLALVIS ATYSMAVSIK MNALLYFPAM MISLFILNDA NVILTLLDLV 251 AMIAWQVAVA VPFLRSFPQQ YLHCAFNFGR KFMYQWSINW QMMDEEAFND 301 KRFHLALLIS HLIALTTLFV TRYPRILPDL WSSLCHPLRK NAVLNANPAK 351 TIPFVLIASN FIGVLFSRSL HYQFLSWYHW TLPILIFWSG MPFFVGPIWY 401 VLHEWCWNSY PPNSQASTLL LALNTVLLLL LALTQLSGSV ALAKSHLRTT 451 SSMEKKLN*

Amino Acid Sequence (or in FASTA format)

       1  MEGEQSPQGE KSLQRKQFVR PPLDLWQDLK DGVRYVIFDC RANLIVMPLL
      51  ILFESMLCKI IIKKVAYTEI DYKAYMEQIE MIQLDGMLDY SQVSGGTGPL
     101  VYPAGHVLIY KMMYWLTEGM DHVERGQVFF RYLYLLTLAL QMACYYLLHL
     151  PPWCVVLACL SKRLHSIYVL RLFNDCFTTL FMVVTVLGAI VASRCHQRPK
     201  LKKSLALVIS ATYSMAVSIK MNALLYFPAM MISLFILNDA NVILTLLDLV
     251  AMIAWQVAVA VPFLRSFPQQ YLHCAFNFGR KFMYQWSINW QMMDEEAFND
     301  KRFHLALLIS HLIALTTLFV TRYPRILPDL WSSLCHPLRK NAVLNANPAK
     351  TIPFVLIASN FIGVLFSRSL HYQFLSWYHW TLPILIFWSG MPFFVGPIWY
     401  VLHEWCWNSY PPNSQASTLL LALNTVLLLL LALTQLSGSV ALAKSHLRTT
     451  SSMEKKLN*

external links for Alg3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Alg3p

1)	Huffaker TC and Robbins PW (1983) Yeast mutants deficient in protein glycosylation. Proc Natl Acad Sci U S A 80(24):7466-70
2)	Kimura T, et al. (1997) A novel yeast gene, RHK1, is involved in the synthesis of the cell wall receptor for the HM-1 killer toxin that inhibits beta-1,3-glucan synthesis. Mol Gen Genet 254(2):139-47
3)	Aebi M, et al. (1996) Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae. Glycobiology 6(4):439-44
4)	Sharma CB, et al. (2001) Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase. Biol Chem 382(2):321-8