ATG8/YBL078C Protein Information Help

Standard Name Atg8p 1
Systematic Name Ybl078cp
Alias Apg8p 2, 3 , Cvt5p 4 , Aut7p 5
ORF Classification Verified
Description Component of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis (10, 11, 12, 3, 6, 7, 8, 9)
Name Description AuTophaGy related 1
Experimental Data
Molecules/cell 2010 13
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 117
Molecular Weight (Da) 13,627
Isoelectric Point (pI) 9.92

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Atg8p (InterPro)
Physical Interactions There are 81 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000036C22 | P38182
MIPS: YBL078C
NCBI: 217035305 | 295789214 | 295982053 | 347447830 | 357380315 | 358009562 | 496705 | 51013191 | 536125 | 586441 | 6319393 | NP_009475.1 | NM_001178318.1
GenBank/EMBL/DDBJ: AAT92889.1 | CAA56032.1 | CAA84899.1 | DAA07045.1 | AY692870 | X79489 | Z35839
Amino Acid Sequence (or in FASTA format)
       1  MKSTFKSEYP FEKRKAESER IADRFKNRIP VICEKAEKSD IPEIDKRKYL
      51  VPADLTVGQF VYVIRKRIML PPEKAIFIFV NDTLPPTAAL MSAIYQEHKD
     101  KDGFLYVTYS GENTFGR*                                   

external links for Atg8p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Atg8p
1) Klionsky DJ, et al.  (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45
2) Kirisako T, et al.  (1999) Formation process of autophagosome is traced with Apg8/Aut7p in yeast. J Cell Biol 147(2):435-46
3) Tsukada M and Ohsumi Y  (1993) Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 333(1-2):169-74
4) Harding TM, et al.  (1995) Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway. J Cell Biol 131(3):591-602
5) Harding TM, et al.  (1996) Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway. J Biol Chem 271(30):17621-4
6) Ichimura Y, et al.  (2000) A ubiquitin-like system mediates protein lipidation. Nature 408(6811):488-92
7) Kirisako T, et al.  (2000) The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway. J Cell Biol 151(2):263-76
8) Nakatogawa H, et al.  (2007) Atg8, a Ubiquitin-like Protein Required for Autophagosome Formation, Mediates Membrane Tethering and Hemifusion. Cell 130(1):165-78
9) Xie Z, et al.  (2008) Atg8 Controls Phagophore Expansion during Autophagosome Formation. Mol Biol Cell 19(8):3290-8
10) Nair U, et al.  (2011) SNARE proteins are required for macroautophagy. Cell 146(2):290-302
11) Kraft C, et al.  (2012) Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy. EMBO J 31(18):3691-703
12) Nair U, et al.  (2012) A role for Atg8-PE deconjugation in autophagosome biogenesis. Autophagy 8(5):780-93
13) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41