ILS1/YBL076C Protein Information Help

Standard Name Ils1p 1
Systematic Name Ybl076cp
ORF Classification Verified
Description Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A (2, 3)
Name Description IsoLeucine-tRNA Synthetase
Gene Product isoleucyl-tRNA synthetase 4
Experimental Data
Molecules/cell 23300 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,072
Molecular Weight (Da) 122,982
Isoelectric Point (pI) 5.94

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ils1p (InterPro)
Physical Interactions There are 35 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052EAA | P09436
MIPS: YBL076C
NCBI: 135134 | 171768 | 3820 | 498991 | 536123 | 6319395 | NP_009477.1
GenBank/EMBL/DDBJ: DAA07046.1 | M19992 | X07886 | X79489 | Z35838
External Classifications EC: 6.1.1.5 [Isoleucine--tRNA ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT
      51  GTPHYGHILA STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG
     101  ITGKDDVFKY GLENYNNECR SIVMTYASDW RKTIGRLGRW IDFDNDYKTM
     151  YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY STGLTTPLSN FEAQQNYKDV
     201  NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD FEYVKIYDET
     251  RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ
     301  FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP
     351  NAIDDLGRFT KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF
     401  CWRSDTPLLY RSVPAWFVRV KNIVPQMLDS VMKSHWVPNT IKEKRFANWI
     451  ANARDWNVSR NRYWGTPIPL WVSDDFEEVV CVGSIKELEE LTGVRNITDL
     501  HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ HYPFENTEKF
     551  DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG
     601  RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV
     651  VSKVLLPWWN SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM
     701  QSLVQFIHEE MGQYKLYTVV PKLLNFIDEL TNWYIRFNRR RLKGENGVED
     751  CLKALNSLFD ALFTFVRAMA PFTPFLSESI YLRLKEYIPE AVLAKYGKDG
     801  RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK KTISLKTPLK
     851  TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW
     901  PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI
     951  RGLPESAVQA GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK
    1001  KCGLEATDDV LVEYELVKDT IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT
    1051  DPIGDEEQSI NDTIFKLKVF KL*

external links for Ils1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Ils1p
1) Nelbock, P., et al.  (1989) ; Personal Communication, Mortimer Map Edition 10
2) Englisch U, et al.  (1987) Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases. Biol Chem Hoppe Seyler 368(8):971-9
3) Miyamoto Y, et al.  (2002) Identification of Saccharomyces cerevisiae isoleucyl-tRNA synthetase as a target of the G1-specific inhibitor Reveromycin A. J Biol Chem 277(32):28810-4
4) Meussdoerffer F and Fink GR  (1983) Structure and expression of two aminoacyl-tRNA synthetase genes from Saccharomyces cerevisiae. J Biol Chem 258(10):6293-9
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41