AST1/YBL069W Protein Information

Standard Name	Ast1p 1
Systematic Name	Ybl069wp
ORF Classification	Verified
Description	Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	ATPase STabilizing 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	429
Molecular Weight (Da)	48,348
Isoelectric Point (pI)	8.57

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ast1p (InterPro)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001261AB \| P35183 MIPS: YBL069W NCBI: 14270687 \| 1870079 \| 1870081 \| 407520 \| 551276 \| 728902 \| NP_009484.2 GenBank/EMBL/DDBJ: DAA07052.1 \| X81843 \| Z26879 \| Z35829 \| Z35831

Amino Acid Sequence (or in FASTA format)
1 MAKDILKNQD PKLQAMIVEH SAPAPKEIPM DAPVLKRVAR PLRHVKFIPI 51 KSLIFHTKTG PMDFSYEKKI KTPIPKNKIV VRVSNVGLNP VDMKIRNGYT 101 SSIYGEIGLG REYSGVITEV GENLNYAWHV GDEVYGIYYH PHLAVGCLQS 151 SILVDPKVDP ILLRPESVSA EEAAGSLFCL ATGYNILNKL SKNKYLKQDS 201 NVLINGGTSS VGMFVIQLLK RHYKLQKKLV IVTSANGPQV LQEKFPDLAD 251 EMIFIDYLTC RGKSSKPLRK MLEEKKISQY DPVEDKETIL NYNEGKFDVV 301 LDFVGGYDIL SHSSSLIHGG GAYVTTVGDY VANYKEDIFD SWDNPSANAR 351 KMFGSIIWSY NYTHYYFDPN AKTASANNDW IEQCGDFLKN GTVKCVVDKV 401 YDWKDHKEAF SYMATQRAQG KLIMNVEKF*

Amino Acid Sequence (or in FASTA format)

       1  MAKDILKNQD PKLQAMIVEH SAPAPKEIPM DAPVLKRVAR PLRHVKFIPI
      51  KSLIFHTKTG PMDFSYEKKI KTPIPKNKIV VRVSNVGLNP VDMKIRNGYT
     101  SSIYGEIGLG REYSGVITEV GENLNYAWHV GDEVYGIYYH PHLAVGCLQS
     151  SILVDPKVDP ILLRPESVSA EEAAGSLFCL ATGYNILNKL SKNKYLKQDS
     201  NVLINGGTSS VGMFVIQLLK RHYKLQKKLV IVTSANGPQV LQEKFPDLAD
     251  EMIFIDYLTC RGKSSKPLRK MLEEKKISQY DPVEDKETIL NYNEGKFDVV
     301  LDFVGGYDIL SHSSSLIHGG GAYVTTVGDY VANYKEDIFD SWDNPSANAR
     351  KMFGSIIWSY NYTHYYFDPN AKTASANNDW IEQCGDFLKN GTVKCVVDKV
     401  YDWKDHKEAF SYMATQRAQG KLIMNVEKF*

external links for Ast1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ast1p

1)	Chang A and Fink GR (1995) Targeting of the yeast plasma membrane [H+]ATPase: a novel gene AST1 prevents mislocalization of mutant ATPase to the vacuole. J Cell Biol 128(1-2):39-49
2)	Bagnat M, et al. (2001) Plasma membrane proton ATPase Pma1p requires raft association for surface delivery in yeast. Mol Biol Cell 12(12):4129-38
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Riveros-Rosas H, et al. (2003) Diversity, taxonomy and evolution of medium-chain dehydrogenase/reductase superfamily. Eur J Biochem 270(16):3309-34