PRS4/YBL068W Protein Information

Standard Name	Prs4p 1
Systematic Name	Ybl068wp
ORF Classification	Verified
Description	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	PhosphoRibosylpyrophosphate Synthetase 5

Experimental Data
Molecules/cell	5280 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	326
Molecular Weight (Da)	35,720
Isoelectric Point (pI)	6.35

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prs4p (InterPro)
Physical Interactions	There are 11 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000158E281 \| P38063 MIPS: YBL068W NCBI: 330443409 \| 341941026 \| 536108 \| 585370 \| 9885239 \| NP_009485.3 GenBank/EMBL/DDBJ: DAA07053.2 \| AJ245726 \| Z35829
External Classifications	EC: 2.7.6.1 [Ribose-phosphate diphosphokinase]

Amino Acid Sequence (or in FASTA format)
1 MNSESREDMA INSIKLLAGN SHPDLAEQIS KKLGIPLSKV GVYQYSNKET 51 SVTIGESLRD EDVYIIQTGI GEQEINDFLM ELLILIHACK IASARKITTV 101 IPNFPYARQD KKDKSRAPIT AKLVANLLQT AGADHVITMD LHASQIQGFF 151 HIPVDNLYAE PSVLNYIRTK TDFDNAILVS PDAGGAKRVA ALADKLDLNF 201 ALIHKERQKA NEVSKMVLVG DVTNKSCLLV DDMADTCGTL VKACDTLMEH 251 GAKEVIAIVT HGIFSGSARE KLRNSRLSRI VCTNTVPVDL DLPIADQIDI 301 SPTFAEAIRR LHNGESVSYL FTHAPV*

Amino Acid Sequence (or in FASTA format)

       1  MNSESREDMA INSIKLLAGN SHPDLAEQIS KKLGIPLSKV GVYQYSNKET
      51  SVTIGESLRD EDVYIIQTGI GEQEINDFLM ELLILIHACK IASARKITTV
     101  IPNFPYARQD KKDKSRAPIT AKLVANLLQT AGADHVITMD LHASQIQGFF
     151  HIPVDNLYAE PSVLNYIRTK TDFDNAILVS PDAGGAKRVA ALADKLDLNF
     201  ALIHKERQKA NEVSKMVLVG DVTNKSCLLV DDMADTCGTL VKACDTLMEH
     251  GAKEVIAIVT HGIFSGSARE KLRNSRLSRI VCTNTVPVDL DLPIADQIDI
     301  SPTFAEAIRR LHNGESVSYL FTHAPV*

external links for Prs4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Prs4p

1)	Carter AT, et al. (1997) PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae. Mol Gen Genet 254(2):148-56
2)	Hernando Y, et al. (1999) Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae. J Biol Chem 274(18):12480-7
3)	Hernando Y, et al. (1998) PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3. J Bacteriol 180(23):6404-7
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Carter AT, et al. (1994) Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae. Yeast 10(8):1031-44
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41