PTC3/YBL056W Protein Information Help

Standard Name Ptc3p 1
Systematic Name Ybl056wp
ORF Classification Verified
Description Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Phosphatase Two C 1
Experimental Data
Molecules/cell 19700 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 468
Molecular Weight (Da) 51,332
Isoelectric Point (pI) 4.23

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ptc3p (InterPro)
Physical Interactions There are 34 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000168EB0 | P34221
MIPS: YBL056W
NCBI: 1622933 | 313742 | 330443415 | 341942260 | 51012959 | 536086 | 84028240 | NP_009497.2
GenBank/EMBL/DDBJ: DAA07064.2 | AY692754 | U72346 | Z23261 | Z35817
External Classifications EC: 3.1.3.16 [Phosphoprotein phosphatase]
Amino Acid Sequence (or in FASTA format)
 
       1  MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES
      51  DEEHLAFYGI FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF
     101  LATDVELLKD EKLKDDHSGC TATVILVSQL KKLLICANSG DSRTVLSTGG
     151  NSKAMSFDHK PTLLSEKSRI VAADGFVEMD RVNGNLALSR AIGDFEFKSN
     201  TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL TSQECVDLVH
     251  YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE
     301  SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL
     351  NRSKDNDDME IDDLDTELGS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ
     401  IRQRPSSDSD GNTSYFHGAS LSDMLASLSN AAAGETEPND ADDNDDNDGE
     451  ENGKNENAKK GSKIEEIE*

external links for Ptc3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Ptc3p
1) Maeda T, et al.  (1993) Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol 13(9):5408-17
2) Cheng A, et al.  (1999) Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev 13(22):2946-57
3) Warmka J, et al.  (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60
4) Young C, et al.  (2002) Role of Ptc2 type 2C Ser/Thr phosphatase in yeast high-osmolarity glycerol pathway inactivation. Eukaryot Cell 1(6):1032-40
5) Leroy C, et al.  (2003) PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break. Mol Cell 11(3):827-35
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41