FUI1/YBL042C Protein Information Help

Standard Name Fui1p
Systematic Name Ybl042cp
ORF Classification Verified
Description High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress (1, 2, 3, 4)
Name Description 5-FlUorourIdine resistance 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 639
Molecular Weight (Da) 72,164
Isoelectric Point (pI) 7.47

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Fui1p (InterPro)
Transmembrane Domains There are 10 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 10 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012AD5B | P38196
MIPS: YBL042C
NCBI: 536059 | 586422 | 6319429 | 872301 | NP_009511.1 | NM_001178282.1
GenBank/EMBL/DDBJ: DAA07076.1 | X78214 | Z35803
External Classifications TC: 2.A.39.3
Amino Acid Sequence (or in FASTA format)
 
       1  MPVSDSGFDN SSKTMKDDTI PTEDYEEITK ESEMGDATKI TSKIDANVIE
      51  KKDTDSENNI TIAQDDEKVS WLQRVVEFFE VKNDSTDLAD HKPENPIRTF
     101  KDLQESLRST YLYNTDLRPV EAKRRTWTWK QYIFFWISGS FNVNTWQISA
     151  TGLQLGLNWW QTWICIWVGY TFVAFFLILG SKVGNNYHIS FPISSRVSFG
     201  IYFSIWIVIN RVVMACVWNS TLAYIGSQCV QLMLKAIFGT NLNTRIKDTI
     251  KNPNLTNFEF MCFMVFWVAC LPFLWFPPDK LRHIFALKSA ITPFAAFGFL
     301  IWTLCKAKGH LALGSLNDNG GAISKTVLAW SVIRAIMSAL DNFSTLILNA
     351  PDFTRFGKTY KSSVYSQLIA LPVCYAIISL IGILSVSAAY TLYGVNYWSP
     401  LDILNRYLDN YTSGNRAGVF LISFIFAFDQ LGANLSGNSI PAGTDLTALL
     451  PKFINIRRGS YICALISLAI CPWDLLSSSS KFTTALAAYA VFLSAIAGVI
     501  SADYFIVRKG YVNIFHCYTD KPGSYYMYNK YGTNWRAVVA YIFGIAPNFA
     551  GFLGSVGVSV PIGAMKVYYL NYFVGYLLAA LSYCILVYFY PIKGIPGDAK
     601  ITDRKWLEEW VEVEEFGTER EAFEEYGGVS TGYEKIRYI*

external links for Fui1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome TCDB

DIP YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Fui1p
1) Wagner R, et al.  (1998) The ORF YBL042 of Saccharomyces cerevisiae encodes a uridine permease. FEMS Microbiol Lett 159(1):69-75
2) Vickers MF, et al.  (2000) Nucleoside transporter proteins of Saccharomyces cerevisiae. Demonstration of a transporter (FUI1) with high uridine selectivity in plasma membranes and a transporter (FUN26) with broad nucleoside selectivity in intracellular membranes. J Biol Chem 275(34):25931-8
3) Zhang J, et al.  (2006) Characterization of the transport mechanism and permeant binding profile of the uridine permease Fui1p of Saccharomyces cerevisiae. J Biol Chem 281(38):28210-21
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Jund R and Lacroute F  (1970) Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae. J Bacteriol 102(3):607-15