URA7/YBL039C Protein Information

Standard Name	Ura7p
Systematic Name	Ybl039cp
ORF Classification	Verified
Description	Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description	URAcil requiring 5

Experimental Data
Molecules/cell	57600 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	579
Molecular Weight (Da)	64,710
Isoelectric Point (pI)	5.93

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ura7p (InterPro)
Physical Interactions	There are 75 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FAD \| P28274 MIPS: YBL039C NCBI: 440583 \| 463269 \| 536053 \| 585767 \| 6319432 \| NP_009514.1 GenBank/EMBL/DDBJ: DAA07080.1 \| X53995 \| X78214 \| Z35800
External Classifications	EC: 6.3.4.2 [CTP synthase]

Amino Acid Sequence (or in FASTA format)
1 MKYVVVSGGV ISGIGKGVLA SSTGMLMKTL GLKVTSIKID PYMNIDAGTM 51 SPLEHGECFV LDDGGETDLD LGNYERYLGV TLTKDHNITT GKIYSHVIAK 101 ERKGDYLGKT VQIVPHLTNA IQDWIERVAK IPVDDTGMEP DVCIIELGGT 151 VGDIESAPFV EALRQFQFKV GKENFALIHV SLVPVIHGEQ KTKPTQAAIK 201 GLRSLGLVPD MIACRCSETL DKPTIDKIAM FCHVGPEQVV NVHDVNSTYH 251 VPLLLLEQKM IDYLHARLKL DEISLTEEEK QRGLELLSKW KATTGNFDES 301 METVKIALVG KYTNLKDSYL SVIKALEHSS MKCRRKLDIK WVEATDLEPE 351 AQESNKTKFH EAWNMVSTAD GILIPGGFGV RGTEGMVLAA RWARENHIPF 401 LGVCLGLQIA TIEFTRSVLG RKDSHSAEFY PDIDEKNHVV VFMPEIDKET 451 MGGSMRLGLR PTFFQNETEW SQIKKLYGDV SEVHERHRHR YEINPKMVDE 501 LENNGLIFVG KDDTGKRCEI LELKNHPYYI ATQYHPEYTS KVLDPSKPFL 551 GLVAASAGIL QDVIEGKYDL EAGENKFNF*

Amino Acid Sequence (or in FASTA format)

       1  MKYVVVSGGV ISGIGKGVLA SSTGMLMKTL GLKVTSIKID PYMNIDAGTM
      51  SPLEHGECFV LDDGGETDLD LGNYERYLGV TLTKDHNITT GKIYSHVIAK
     101  ERKGDYLGKT VQIVPHLTNA IQDWIERVAK IPVDDTGMEP DVCIIELGGT
     151  VGDIESAPFV EALRQFQFKV GKENFALIHV SLVPVIHGEQ KTKPTQAAIK
     201  GLRSLGLVPD MIACRCSETL DKPTIDKIAM FCHVGPEQVV NVHDVNSTYH
     251  VPLLLLEQKM IDYLHARLKL DEISLTEEEK QRGLELLSKW KATTGNFDES
     301  METVKIALVG KYTNLKDSYL SVIKALEHSS MKCRRKLDIK WVEATDLEPE
     351  AQESNKTKFH EAWNMVSTAD GILIPGGFGV RGTEGMVLAA RWARENHIPF
     401  LGVCLGLQIA TIEFTRSVLG RKDSHSAEFY PDIDEKNHVV VFMPEIDKET
     451  MGGSMRLGLR PTFFQNETEW SQIKKLYGDV SEVHERHRHR YEINPKMVDE
     501  LENNGLIFVG KDDTGKRCEI LELKNHPYYI ATQYHPEYTS KVLDPSKPFL
     551  GLVAASAGIL QDVIEGKYDL EAGENKFNF*

external links for Ura7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Ura7p

1)	Yang WL, et al. (1994) Purification and characterization of CTP synthetase, the product of the URA7 gene in Saccharomyces cerevisiae. Biochemistry 33(35):10785-93
2)	Ozier-Kalogeropoulos O, et al. (1994) Use of synthetic lethal mutants to clone and characterize a novel CTP synthetase gene in Saccharomyces cerevisiae. Mol Gen Genet 242(4):431-9
3)	Nadkarni AK, et al. (1995) Differential biochemical regulation of the URA7- and URA8-encoded CTP synthetases from Saccharomyces cerevisiae. J Biol Chem 270(42):24982-8
4)	Ostrander DB, et al. (1998) Effect of CTP synthetase regulation by CTP on phospholipid synthesis in Saccharomyces cerevisiae. J Biol Chem 273(30):18992-9001
5)	Ozier-Kalogeropoulos O, et al. (1991) Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase. Mol Gen Genet 231(1):7-16
6)	McDonough VM, et al. (1995) Regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by CTP. J Biol Chem 270(32):18774-80
7)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8)	Noree C, et al. (2010) Identification of novel filament-forming proteins in Saccharomyces cerevisiae and Drosophila melanogaster. J Cell Biol 190(4):541-51
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41