STU1/YBL034C Protein Information

Standard Name	Stu1p
Systematic Name	Ybl034cp
ORF Classification	Verified
Description	Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles (1, 2)
Name Description	Suppressor of TUb2 mutation

Experimental Data
Molecules/cell	521 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,513
Molecular Weight (Da)	174,176
Isoelectric Point (pI)	6.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Stu1p (InterPro)
Physical Interactions	There are 19 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136157 \| P38198 MIPS: YBL034C NCBI: 511142 \| 525278 \| 536043 \| 586418 \| 6319437 \| NP_009519.1 GenBank/EMBL/DDBJ: DAA07085.1 \| X74738 \| Z35655 \| Z35795

Amino Acid Sequence (or in FASTA format)
1 MSSFNNETNN NSNTNTHPDD SFPLYTVFKD ESVPIEEKMA LLTRFKGHVK 51 KELVNESSIQ AYFAALLFIS GHYAYRSYPR LIFLSHSSLC YLIKRVAMQS 101 PVQFNDTLVE QLLNHLIFEL PNEKKFWLAS IKAIEAIYLV NPSKIQAILA 151 NFLRRPSENQ NGDYLNRIKS TLLTIDELIQ INEKNNSNHL QLLRFFMLSF 201 TNLLNNNLNE HANDDNNNVI IELIFDIMYK YLKMDDENSQ DLIDGFINDL 251 EVEKFKQKFI SLAKSQDQHG SQEDKSTLFD EEYEFQLLLA EAKLPQLSNN 301 LSSKDPAMKK NYESLNQLQQ DLENLLAPFQ SVKETEQNWK LRQSNIIELD 351 NIISGNIPKD NPEEFVTVIK EVQLIELISR ATSSLRTTLS LTALLFLKRL 401 IHILNDQLPL SILDQIFVIF KNLLSSTKKI SSQTAFHCLI TLIIDINHFH 451 NKLFQLSFLL INEKTVTPRF CSAILLRSFL IKFNDSNLSL NNSNTTSPTS 501 KLENNIIYIE EWLKKGISDS QTTVREAMRL TFWYFYKCYP TNAKRLLSSS 551 FSPQLKKATE LAIPAHLNIN YQVSRVSSTA SASSATSRLY SHSSNNSSRK 601 TSLLEQKRNY PSYAQPTQSS STSLLNAPAV TAGGSVIASK LSNKLKTNLR 651 STSEYSSKEN EKRARHHDSM NSVSNSNTKD NNNVTKRKVS APPSSTAATK 701 VSENYTNFDD FPSNQIDLTD ELSNSYSNPL IKKYMDKNDV SMSSSPISLK 751 GSNKLGEYET LYKKFNDASF PAQIKDALQY LQKELLLTSQ QSSSAPKFEF 801 PMIMKKLRQI MIKSPNDFKP FLSIEKFTNG VPLNYLIELY SINSFDYAEI 851 LKNRMNPEKP YELTNLIITI ADLFNFLNAN NCPNDFKLYY MKYKTTFFNY 901 NFKLLLEIFR NLNIKHDNTL RSGTNDLMPK ISMILFQIYG KEFDYTCYFN 951 LIFEIYKFDN NRFNKLLADF DIVSTKMKIC HELEKKDANF KVEDIISRES 1001 SVSFTPIDNK KSEGDEESDD AVDENDVKKC MEMTMINPFK NLETDKTLEL 1051 KNNVGKRTSS TDSVVIHDDN DKDKKLSEMT KIVSVYQLDQ PNPAKEEDDI 1101 DMENSQKSDL NLSEIFQNSG ENTERKLKDD NEPTVKFSTD PPKIINEPEK 1151 LIGNGNENEK PDLETMSPIK INGDENMGQK QRITVKRERD VALTEQDINS 1201 KKMKLVNNKK SEKMHLLIMD NFPRDSLTVY EISHLLMVDS NGNTLMDFDV 1251 YFNHMSKAIN RIKSGSFTMK HINYLIEPLI TCFQNQKMTD WLTNENGFDE 1301 LLDVAIMLLK STDDTPSIPS KISSKSIILV HCLLVWKKFL NTLSENADDD 1351 GVSVRMCFEE VWEQILLMLN KFSDYGNEIY KLAQEFRDSL MLSHFFKKHS 1401 ATRILSMLVT EIQPDTAGVK ETFLIETLWK MLQSPTICQQ FKKSNISEII 1451 QTMSYFIMGT DNTSWNFTSA VVLARCLRVL QTTPDYTEQE TERLFDCLPK 1501 NVFKMIMFIA SNE*

Amino Acid Sequence (or in FASTA format)

       1  MSSFNNETNN NSNTNTHPDD SFPLYTVFKD ESVPIEEKMA LLTRFKGHVK
      51  KELVNESSIQ AYFAALLFIS GHYAYRSYPR LIFLSHSSLC YLIKRVAMQS
     101  PVQFNDTLVE QLLNHLIFEL PNEKKFWLAS IKAIEAIYLV NPSKIQAILA
     151  NFLRRPSENQ NGDYLNRIKS TLLTIDELIQ INEKNNSNHL QLLRFFMLSF
     201  TNLLNNNLNE HANDDNNNVI IELIFDIMYK YLKMDDENSQ DLIDGFINDL
     251  EVEKFKQKFI SLAKSQDQHG SQEDKSTLFD EEYEFQLLLA EAKLPQLSNN
     301  LSSKDPAMKK NYESLNQLQQ DLENLLAPFQ SVKETEQNWK LRQSNIIELD
     351  NIISGNIPKD NPEEFVTVIK EVQLIELISR ATSSLRTTLS LTALLFLKRL
     401  IHILNDQLPL SILDQIFVIF KNLLSSTKKI SSQTAFHCLI TLIIDINHFH
     451  NKLFQLSFLL INEKTVTPRF CSAILLRSFL IKFNDSNLSL NNSNTTSPTS
     501  KLENNIIYIE EWLKKGISDS QTTVREAMRL TFWYFYKCYP TNAKRLLSSS
     551  FSPQLKKATE LAIPAHLNIN YQVSRVSSTA SASSATSRLY SHSSNNSSRK
     601  TSLLEQKRNY PSYAQPTQSS STSLLNAPAV TAGGSVIASK LSNKLKTNLR
     651  STSEYSSKEN EKRARHHDSM NSVSNSNTKD NNNVTKRKVS APPSSTAATK
     701  VSENYTNFDD FPSNQIDLTD ELSNSYSNPL IKKYMDKNDV SMSSSPISLK
     751  GSNKLGEYET LYKKFNDASF PAQIKDALQY LQKELLLTSQ QSSSAPKFEF
     801  PMIMKKLRQI MIKSPNDFKP FLSIEKFTNG VPLNYLIELY SINSFDYAEI
     851  LKNRMNPEKP YELTNLIITI ADLFNFLNAN NCPNDFKLYY MKYKTTFFNY
     901  NFKLLLEIFR NLNIKHDNTL RSGTNDLMPK ISMILFQIYG KEFDYTCYFN
     951  LIFEIYKFDN NRFNKLLADF DIVSTKMKIC HELEKKDANF KVEDIISRES
    1001  SVSFTPIDNK KSEGDEESDD AVDENDVKKC MEMTMINPFK NLETDKTLEL
    1051  KNNVGKRTSS TDSVVIHDDN DKDKKLSEMT KIVSVYQLDQ PNPAKEEDDI
    1101  DMENSQKSDL NLSEIFQNSG ENTERKLKDD NEPTVKFSTD PPKIINEPEK
    1151  LIGNGNENEK PDLETMSPIK INGDENMGQK QRITVKRERD VALTEQDINS
    1201  KKMKLVNNKK SEKMHLLIMD NFPRDSLTVY EISHLLMVDS NGNTLMDFDV
    1251  YFNHMSKAIN RIKSGSFTMK HINYLIEPLI TCFQNQKMTD WLTNENGFDE
    1301  LLDVAIMLLK STDDTPSIPS KISSKSIILV HCLLVWKKFL NTLSENADDD
    1351  GVSVRMCFEE VWEQILLMLN KFSDYGNEIY KLAQEFRDSL MLSHFFKKHS
    1401  ATRILSMLVT EIQPDTAGVK ETFLIETLWK MLQSPTICQQ FKKSNISEII
    1451  QTMSYFIMGT DNTSWNFTSA VVLARCLRVL QTTPDYTEQE TERLFDCLPK
    1501  NVFKMIMFIA SNE*

external links for Stu1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Stu1p

1)	Pasqualone D and Huffaker TC (1994) STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle. J Cell Biol 127(6 Pt 2):1973-84
2)	Yin H, et al. (2002) Stu1p is physically associated with beta-tubulin and is required for structural integrity of the mitotic spindle. Mol Biol Cell 13(6):1881-92
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41