NCL1/YBL024W Protein Information Help

Standard Name Ncl1p 1
Systematic Name Ybl024wp
Alias Trm4p
ORF Classification Verified
Description S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 (1, 2, 3)
Name Description NuCLear protein 1
Experimental Data
Molecules/cell 16100 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 684
Molecular Weight (Da) 77,878
Isoelectric Point (pI) 7.32

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ncl1p (InterPro)
Physical Interactions There are 35 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053289 | P38205
MIPS: YBL024W
NCBI: 536023 | 586408 | 602895 | 6319447 | NP_009529.1
GenBank/EMBL/DDBJ: DAA07096.1 | X77291 | Z35785
External Classifications EC: 2.1.1.202 [Multisite-specific tRNA:(cytosine-C(5))-methyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MARRKNFKKG NKKTFGARDD SRAQKNWSEL VKENEKWEKY YKTLALFPED
      51  QWEEFKKTCQ APLPLTFRIT GSRKHAGEVL NLFKERHLPN LTNVEFEGEK
     101  IKAPVELPWY PDHLAWQLDV PKTVIRKNEQ FAKTQRFLVV ENAVGNISRQ
     151  EAVSMIPPIV LEVKPHHTVL DMCAAPGSKT AQLIEALHKD TDEPSGFVVA
     201  NDADARRSHM LVHQLKRLNS ANLMVVNHDA QFFPRIRLHG NSNNKNDVLK
     251  FDRILCDVPC SGDGTMRKNV NVWKDWNTQA GLGLHAVQLN ILNRGLHLLK
     301  NNGRLVYSTC SLNPIENEAV VAEALRKWGD KIRLVNCDDK LPGLIRSKGV
     351  SKWPVYDRNL TEKTKGDEGT LDSFFSPSEE EASKFNLQNC MRVYPHQQNT
     401  GGFFITVFEK VEDSTEAATE KLSSETPALE SEGPQTKKIK VEEVQKKERL
     451  PRDANEEPFV FVDPQHEALK VCWDFYGIDN IFDRNTCLVR NATGEPTRVV
     501  YTVCPALKDV IQANDDRLKI IYSGVKLFVS QRSDIECSWR IQSESLPIMK
     551  HHMKSNRIVE ANLEMLKHLL IESFPNFDDI RSKNIDNDFV EKMTKLSSGC
     601  AFIDVSRNDP AKENLFLPVW KGNKCINLMV CKEDTHELLY RIFGIDANAK
     651  ATPSAEEKEK EKETTESPAE TTTGTSTEAP SAAN*

external links for Ncl1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ncl1p
1) Wu P, et al.  (1998) NCL1, a novel gene for a non-essential nuclear protein in Saccharomyces cerevisiae. Gene 220(1-2):109-17
2) Motorin Y and Grosjean H  (1999) Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme. RNA 5(8):1105-18
3) Chan CT, et al.  (2012) Reprogramming of tRNA modifications controls the oxidative stress response by codon-biased translation of proteins. Nat Commun 3():937
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41