FUS3/YBL016W Protein Information

Standard Name	Fus3p
Systematic Name	Ybl016wp
Alias	Dac2p 1
ORF Classification	Verified
Description	Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its degradation (2, 3, 4, 5, 6, 7)
Name Description	cell FUSion

Experimental Data
Molecules/cell	8480 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	353
Molecular Weight (Da)	40,772
Isoelectric Point (pI)	7.11

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fus3p (InterPro)
Physical Interactions	There are 218 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012AD91 \| P16892 MIPS: YBL016W NCBI: 119389443 \| 171533 \| 3304 \| 417014 \| 51013643 \| 510447 \| 536007 \| 6319455 \| 85544238 \| 85544239 \| 85544241 \| 85544243 \| 93279425 \| 93279426 \| 93279478 \| 93279479 \| NP_009537.1 GenBank/EMBL/DDBJ: DAA07104.1 \| AY693096 \| M31132 \| X68577 \| X69572 \| Z35777
External Classifications	EC: 2.7.11.24 [Mitogen-activated protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MPKRIVYNIS SDFQLKSLLG EGAYGVVCSA THKPTGEIVA IKKIEPFDKP 51 LFALRTLREI KILKHFKHEN IITIFNIQRP DSFENFNEVY IIQELMQTDL 101 HRVISTQMLS DDHIQYFIYQ TLRAVKVLHG SNVIHRDLKP SNLLINSNCD 151 LKVCDFGLAR IIDESAADNS EPTGQQSGMT EYVATRWYRA PEVMLTSAKY 201 SRAMDVWSCG CILAELFLRR PIFPGRDYRH QLLLIFGIIG TPHSDNDLRC 251 IESPRAREYI KSLPMYPAAP LEKMFPRVNP KGIDLLQRML VFDPAKRITA 301 KEALEHPYLQ TYHDPNDEPE GEPIPPSFFE FDHYKEALTT KDLKKLIWNE 351 IFS*

Amino Acid Sequence (or in FASTA format)

       1  MPKRIVYNIS SDFQLKSLLG EGAYGVVCSA THKPTGEIVA IKKIEPFDKP
      51  LFALRTLREI KILKHFKHEN IITIFNIQRP DSFENFNEVY IIQELMQTDL
     101  HRVISTQMLS DDHIQYFIYQ TLRAVKVLHG SNVIHRDLKP SNLLINSNCD
     151  LKVCDFGLAR IIDESAADNS EPTGQQSGMT EYVATRWYRA PEVMLTSAKY
     201  SRAMDVWSCG CILAELFLRR PIFPGRDYRH QLLLIFGIIG TPHSDNDLRC
     251  IESPRAREYI KSLPMYPAAP LEKMFPRVNP KGIDLLQRML VFDPAKRITA
     301  KEALEHPYLQ TYHDPNDEPE GEPIPPSFFE FDHYKEALTT KDLKKLIWNE
     351  IFS*

external links for Fus3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Fus3p

1)	Fujimura HA (1992) The DAC2/FUS3 protein kinase is not essential for transcriptional activation of the mating pheromone response pathway in Saccharomyces cerevisiae. Mol Gen Genet 235(2-3):450-2
2)	Elion EA, et al. (1993) FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell 4(5):495-510
3)	Garrison TR, et al. (1999) Feedback phosphorylation of an RGS protein by MAP kinase in yeast. J Biol Chem 274(51):36387-91
4)	Breitkreutz A and Tyers M (2002) MAPK signaling specificity: it takes two to tango. Trends Cell Biol 12(6):254-7
5)	Ptashne M and Gann A (2003) Signal transduction. Imposing specificity on kinases. Science 299(5609):1025-7
6)	Matheos D, et al. (2004) Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p. J Cell Biol 165(1):99-109
7)	Bao MZ, et al. (2004) Pheromone-dependent destruction of the Tec1 transcription factor is required for MAP kinase signaling specificity in yeast. Cell 119(7):991-1000
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41