HIR1/YBL008W Protein Information

Standard Name	Hir1p
Systematic Name	Ybl008wp
ORF Classification	Verified
Description	Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores (1, 2, 3, 4, 5, 6, 7)
Name Description	HIstone Regulation 3, 8

Experimental Data
Molecules/cell	846 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	840
Molecular Weight (Da)	93,858
Isoelectric Point (pI)	7.16

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hir1p (InterPro)
Physical Interactions	There are 41 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD23 \| P32479 MIPS: YBL008W NCBI: 1680406 \| 330443419 \| 341941072 \| 417122 \| 535992 \| NP_009545.2 GenBank/EMBL/DDBJ: DAA07112.2 \| L03838 \| S47695 \| Z35769

Amino Acid Sequence (or in FASTA format)
1 MKVVKFPWLA HREESRKYEI YTVDVSHDGK RLATGGLDGK IRIWSIDSIL 51 RCMELESLTP EIPLPQDLQM PLCSMSRHTG SITCVKFSPD GKYLASGSDD 101 RILLIWALDE EQSSQPAFGS EHEREHWTVR KRLVAHDNDI QDICWAPDSS 151 ILVTVGLDRS VIVWNGSTFE KLKRFDVHQS LVKGVVFDPA NKYFATTSDD 201 RTMKIFRYHK TGDISFTIEH IITEPFKESP LTTYFRRPSW SPDGQHIAVP 251 NATNGPVSSV AIVNRGTWDT NVSLIGHDAP TEVARFNPRL FERNAGVKQK 301 KDDDPENALV GQNDDKVHHF DKNIDSVVAT AGQDKSLAVW STSRPRPILV 351 AFDIANKSIT DMSWNPDGSL LFVASLDSSI TLFKFENNEL GKPIPLEKNM 401 EQLYRYGVDK DSLDFPESIN QLLLEDQTKS FKHTKISTSK LGENHPTLAT 451 NSASNQKDNN DASVSRSEHI NILIPKRKKD AILNKAVTLK SGKKRVAPTL 501 ISTSSSSPFS NGIKKPTLDS KRIENNVKSS TKTINSKNTL LNVPEGVEKK 551 ISISSFPLPR LGIHSLIMGT KERSAWKISN SELENDDADN AGGKGSDGTS 601 NSIDDIAVLS EEENDFHRMT LNAKLTQEKI WSEEPTTRCL LQSDVIPDTD 651 VVVLEGGSLD DIAVLEIRNG VERSIQFDSE ALLDNPTRIL GYQGGKRTIE 701 TFIPEVIICA IGSKDCKCWC LASANGSIYI LSYNGQQRIP KICLGHKVIK 751 MVTSSKYLLV LTERGLFFAW DLLDLKLVLR NVPILPILNG QPIHGNKVRI 801 NKVIKCFRLD GSSCDLLLEV GDPKNVYKWT KDLGCWSLYK *

Amino Acid Sequence (or in FASTA format)

       1  MKVVKFPWLA HREESRKYEI YTVDVSHDGK RLATGGLDGK IRIWSIDSIL
      51  RCMELESLTP EIPLPQDLQM PLCSMSRHTG SITCVKFSPD GKYLASGSDD
     101  RILLIWALDE EQSSQPAFGS EHEREHWTVR KRLVAHDNDI QDICWAPDSS
     151  ILVTVGLDRS VIVWNGSTFE KLKRFDVHQS LVKGVVFDPA NKYFATTSDD
     201  RTMKIFRYHK TGDISFTIEH IITEPFKESP LTTYFRRPSW SPDGQHIAVP
     251  NATNGPVSSV AIVNRGTWDT NVSLIGHDAP TEVARFNPRL FERNAGVKQK
     301  KDDDPENALV GQNDDKVHHF DKNIDSVVAT AGQDKSLAVW STSRPRPILV
     351  AFDIANKSIT DMSWNPDGSL LFVASLDSSI TLFKFENNEL GKPIPLEKNM
     401  EQLYRYGVDK DSLDFPESIN QLLLEDQTKS FKHTKISTSK LGENHPTLAT
     451  NSASNQKDNN DASVSRSEHI NILIPKRKKD AILNKAVTLK SGKKRVAPTL
     501  ISTSSSSPFS NGIKKPTLDS KRIENNVKSS TKTINSKNTL LNVPEGVEKK
     551  ISISSFPLPR LGIHSLIMGT KERSAWKISN SELENDDADN AGGKGSDGTS
     601  NSIDDIAVLS EEENDFHRMT LNAKLTQEKI WSEEPTTRCL LQSDVIPDTD
     651  VVVLEGGSLD DIAVLEIRNG VERSIQFDSE ALLDNPTRIL GYQGGKRTIE
     701  TFIPEVIICA IGSKDCKCWC LASANGSIYI LSYNGQQRIP KICLGHKVIK
     751  MVTSSKYLLV LTERGLFFAW DLLDLKLVLR NVPILPILNG QPIHGNKVRI
     801  NKVIKCFRLD GSSCDLLLEV GDPKNVYKWT KDLGCWSLYK *

external links for Hir1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Hir1p

1)	DeSilva H, et al. (1998) Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Genetics 148(2):657-67
2)	Kaufman PD, et al. (1998) Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I. Mol Cell Biol 18(8):4793-806
3)	Sherwood PW, et al. (1993) Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(1):28-38
4)	Spector MS, et al. (1997) Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle. Mol Cell Biol 17(2):545-52
5)	Sharp JA, et al. (2001) Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol 11(7):463-73
6)	Sharp JA, et al. (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
7)	Prochasson P, et al. (2005) The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev 19(21):2534-9
8)	Osley MA and Lycan D (1987) Trans-acting regulatory mutations that alter transcription of Saccharomyces cerevisiae histone genes. Mol Cell Biol 7(12):4204-10
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41