CDC15/YAR019C Protein Information Help

Standard Name Cdc15p 1
Systematic Name Yar019cp
Alias Lyt1p
ORF Classification Verified
Description Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress (2, 3, 4)
Name Description Cell Division Cycle 5
Experimental Data
Molecules/cell 238 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 974
Molecular Weight (Da) 110,241
Isoelectric Point (pI) 8

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cdc15p (InterPro)
Physical Interactions There are 65 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000158F711 | P27636
MIPS: YAR019C
NCBI: 171001 | 298025 | 298030 | 330443388 | 341940540 | 349757 | 399197 | NP_009411.2
GenBank/EMBL/DDBJ: DAA06996.2 | L22015 | M67445 | X52683 | X60549
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MNSMADTDRV NLTPIQRASE KSVQYHLKQV IGRGSYGVVY KAINKHTDQV
      51  VAIKEVVYEN DEELNDIMAE ISLLKNLNHN NIVKYHGFIR KSYELYILLE
     101  YCANGSLRRL ISRSSTGLSE NESKTYVTQT LLGLKYLHGE GVIHRDIKAA
     151  NILLSADNTV KLADFGVSTI VNSSALTLAG TLNWMAPEIL GNRGASTLSD
     201  IWSLGATVVE MLTKNPPYHN LTDANIYYAV ENDTYYPPSS FSEPLKDFLS
     251  KCFVKNMYKR PTADQLLKHV WINSTENVKV DKLNKFKEDF TDADYHWDAD
     301  FQEEKLNISP SKFSLAAAPA AWAENNQELD LMPPTESQLL SQLKSSSKPL
     351  TDLHVLFSVC SLENIADTII ECLSRTTVDK RLITAFGSIF VYDTQHNHSR
     401  LRLKFIAMGG IPLIIKFEHL AKEFVIDYPQ TLIECGIMYP PNFASLKTPK
     451  YILELVYRFY DLTSTAFWCR WCFKHLDISL LLNNIHERRA QSILLKLSSY
     501  APWSFEKILP SLIDSKLKKK ILISPQITYV VFKSINYMIT TNDDKIHKSA
     551  IPSSSSLPLS SSPTRNSPVN SVQSPSRSPV HSLMATRPSS PMRHKSISNF
     601  PHLTISSKSR LLIELPEGFF TWLTSFFVDM AQIKDLSVLK YFTKLCYLTV
     651  HINSTFLNDL LDNDAFFAFI RNIDTIIPFI DDAKTAAFIW KQITAICVEM
     701  SLDMDQMSAS LFSTAMNFIR KKNNTSISGL EIILNCLHFT LRNVNDDVAP
     751  TVGSSESHSV FLIKVNNDAA IELPIDQLVD LFYALNDDDV NLSKLISIFT
     801  KICSLPGFEN LTINIIFHPN FYEKIVSFFD TYFNSLLIQI DLLKFIKLIF
     851  SKSLLKLYDY TGQPDPIKQT EPNRRNKATV FKLRAILVQI TEFLNNNWNK
     901  DVPKRNSNQV GGDSVLICQL CEDIRSLSKK GSLQKVSSVT AAIGSSPTKD
     951  ERSNLRSSKD KSDGFSVPIT TFQT*

external links for Cdc15p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Cdc15p
1) Cid, V.J. and M.Sanchez  (1997) LYT1 is allelic to CDC15. Unpublished results
2) Bardin AJ, et al.  (2003) Mitotic exit regulation through distinct domains within the protein kinase Cdc15. Mol Cell Biol 23(14):5018-30
3) Pablo-Hernando ME, et al.  (2007) Cdc15 is required for spore morphogenesis independently of Cdc14 in Saccharomyces cerevisiae. Genetics 177(1):281-93
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41