ADE1/YAR015W Protein Information

Standard Name	Ade1p 1
Systematic Name	Yar015wp
ORF Classification	Verified
Description	N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description	ADEnine requiring 1

Experimental Data
Molecules/cell	4280 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	306
Molecular Weight (Da)	34,603
Isoelectric Point (pI)	5.8

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ade1p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001682A4 \| P27616 MIPS: YAR015W NCBI: 131634 \| 157879485 \| 170996 \| 170999 \| 308153480 \| 313507122 \| 313507250 \| 313507251 \| 313507252 \| 349755 \| 4043 \| 408489437 \| 6319326 \| NP_009409.1 GenBank/EMBL/DDBJ: DAA06994.1 \| L22015 \| M61209 \| M67445 \| X60549
External Classifications	EC: 6.3.2.6 [Phosphoribosylaminoimidazolesuccinocarboxamide synthase]

Amino Acid Sequence (or in FASTA format)
1 MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI 51 PEKGILLTKL SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT 101 QLEDRSLLVH KHKLIPLEVI VRGYITGSAW KEYVKTGTVH GLKQPQGLKE 151 SQEFPEPIFT PSTKAEQGEH DENISPAQAA ELVGEDLSRR VAELAVKLYS 201 KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS SRFWNGASYK 251 VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT 301 GSKWSH*

Amino Acid Sequence (or in FASTA format)

       1  MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI
      51  PEKGILLTKL SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT
     101  QLEDRSLLVH KHKLIPLEVI VRGYITGSAW KEYVKTGTVH GLKQPQGLKE
     151  SQEFPEPIFT PSTKAEQGEH DENISPAQAA ELVGEDLSRR VAELAVKLYS
     201  KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS SRFWNGASYK
     251  VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT
     301  GSKWSH*

external links for Ade1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Ade1p

1)	Dorfman BZ (1969) The isolation of adenylosuccinate synthetase mutants in yeast by selection for constitutive behavior in pigmented strains. Genetics 61(2):377-89
2)	Myasnikov AN, et al. (1991) The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control. Gene 109(1):143-7
3)	Jones EW and Fink GR (1982) "Regulation of amino acid and nucleotide biosynthesis in yeast." Pp.181-299 in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, edited by Strathern JN, Jones EW and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41