GDH3/YAL062W Protein Information

Standard Name	Gdh3p
Systematic Name	Yal062wp
Alias	Fun51p
ORF Classification	Verified
Description	NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Glutamate DeHydrogenase

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	457
Molecular Weight (Da)	49,627
Isoelectric Point (pI)	5.23

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gdh3p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012930B \| P39708 MIPS: YAL062W NCBI: 595524 \| 6319256 \| 729323 \| NP_009339.1 \| NM_001178204.1 GenBank/EMBL/DDBJ: DAA06927.1 \| U12980
External Classifications	EC: 1.4.1.4 [Glutamate dehydrogenase (NADP(+))]

Amino Acid Sequence (or in FASTA format)
1 MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW 51 ENDNGEQEVA QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN 101 ALTGLDMGGG KGGLCVDLKG KSDNEIRRIC YAFMRELSRH IGKDTDVPAG 151 DIGVGGREIG YLFGAYRSYK NSWEGVLTGK GLNWGGSLIR PEATGFGLVY 201 YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL GGIVVSLSDS 251 KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR 301 PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS 351 VFETARSTAT NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV 401 DQELKKIMIN CFNDCIQAAQ EYSTEKNTNT LPSLVKGANI ASFVMVADAM 451 LDQGDVF*

Amino Acid Sequence (or in FASTA format)

       1  MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW
      51  ENDNGEQEVA QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN
     101  ALTGLDMGGG KGGLCVDLKG KSDNEIRRIC YAFMRELSRH IGKDTDVPAG
     151  DIGVGGREIG YLFGAYRSYK NSWEGVLTGK GLNWGGSLIR PEATGFGLVY
     201  YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL GGIVVSLSDS
     251  KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR
     301  PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS
     351  VFETARSTAT NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV
     401  DQELKKIMIN CFNDCIQAAQ EYSTEKNTNT LPSLVKGANI ASFVMVADAM
     451  LDQGDVF*

external links for Gdh3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Gdh3p

1)	Avendano A, et al. (1997) GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. J Bacteriol 179(17):5594-7
2)	DeLuna A, et al. (2001) NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles. J Biol Chem 276(47):43775-83
3)	Riego L, et al. (2002) GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth. Biochem Biophys Res Commun 293(1):79-85
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61