PTA1/YAL043C Protein Information Help

Standard Name Pta1p
Systematic Name Yal043cp
Alias Fun39p
ORF Classification Verified
Description Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII (1, 2, 3, 4, 5)
Name Description Pre-Trna Accumulation 2
Experimental Data
Molecules/cell 3730 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 785
Molecular Weight (Da) 88,472
Isoelectric Point (pI) 6.17

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pta1p (InterPro)
Physical Interactions There are 194 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052DDD | Q01329
MIPS: YAL043C
NCBI: 172290 | 266859 | 595541 | 6319273 | NP_009356.1
GenBank/EMBL/DDBJ: DAA06943.1 | M95673 | U12980
Amino Acid Sequence (or in FASTA format)
 
       1  MSSAEMEQLL QAKTLAMHNN PTEMLPKVLE TTASMYHNGN LSKLKLPLAK
      51  FFTQLVLDVV SMDSPIANTE RPFIAAQYLP LLLAMAQSTA DVLVYKNIVL
     101  IMCASYPLVL DLVAKTSNQE MFDQLCMLKK FVLSHWRTAY PLRATVDDET
     151  DVEQWLAQID QNIGVKLATI KFISEVVLSQ TKSPSGNEIN SSTIPDNHPV
     201  LNKPALESEA KRLLDMLLNY LIEEQYMVSS VFIGIINSLS FVIKRRPQTT
     251  IRILSGLLRF NVDAKFPLEG KSDLNYKLSK RFVERAYKNF VQFGLKNQII
     301  TKSLSSGSGS SIYSKLTKIS QTLHVIGEET KSKGILNFDP SKGNSKKTLS
     351  RQDKLKYISL WKRQLSALLS TLGVSTKTPT PVSAPATGSS TENMLDQLKI
     401  LQKYTLNKAS HQGNTFFNNS PKPISNTYSS VYSLMNSSNS NQDVTQLPND
     451  ILIKLSTEAI LQMDSTKLIT GLSIVASRYT DLMNTYINSV PSSSSSKRKS
     501  DDDDDGNDNE EVGNDGPTAN SKKIKMETEP LAEEPEEPED DDRMQKMLQE
     551  EESAQEISGD ANKSTSAIKE IAPPFEPDSL TQDEKLKYLS KLTKKLFELS
     601  GRQDTTRAKS SSSSSILLDD DDSSSWLHVL IRLVTRGIEA QEASDLIREE
     651  LLGFFIQDFE QRVSLIIEWL NEEWFFQTSL HQDPSNYKKW SLRVLESLGP
     701  FLENKHRRFF IRLMSELPSL QSDHLEALKP ICLDPARSSL GFQTLKFLIM
     751  FRPPVQDTVR DLLHQLKQED EGLHKQCDSL LDRLK*

external links for Pta1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pta1p
1) Preker PJ, et al.  (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37
2) O'Connor JP and Peebles CL  (1992) PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing. Mol Cell Biol 12(9):3843-56
3) Zhao J, et al.  (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40
4) Rodriguez CR, et al.  (2000) Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol 20(1):104-12
5) Nedea E, et al.  (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41